孕期钙、维生素D和抗坏血酸联合干预对铅暴露大鼠骨铅动员的影响  

Effects of combined intervention of calcium,vitamin D,and ascorbic acid during pregnancy on bone lead mobilization in rats exposed to lead

作　　者：张林[1] 卢安心 刘军霞[1] 李菁 林崟 颜崇淮[1] ZHANG Lin;LU An-xin;LIU Jun-xia;LI Jing;LIN Yun;YAN Chong-huai(Key Laboratory of Environment and Child Health,Xinhua Hospital,Medical College of Shanghai Jiao Tong University,Yangpu District,Shanghai 200092,China;不详)

机构地区：[1]上海交通大学医学院附属新华医院环境与儿童健康重点实验室,上海200092 [2]上海交通大学公共卫生学院

出　　处：《现代预防医学》2023年第15期2823-2828,2838,共7页Modern Preventive Medicine

基　　金：国家自然科学基金(81973062)。

摘　　要：目的 观察孕期补充钙、维生素D(VD)和抗坏血酸(Asc)三种营养素对骨铅动员的影响。方法 38只刚断乳Wistar雌性大鼠随机分为对照组(0.05%醋酸钠)和铅暴露组(0.05%醋酸铅),饮水染毒4周后改饮蒸馏水。经4周洗脱期后,雌雄合笼,将铅暴露组受孕成功的大鼠随机分为铅组和干预组,后者给予0.02%钙、100μg/L VD和0.2%Asc混合水溶液,其余组继续饮用蒸馏水,在孕3、10和17 d(GD3、10、17)时收集血和骨样本。电感耦合等离子体质谱仪(ICP-MS)检测孕鼠血铅和骨铅水平并计算铅排泄率;微计算机断层扫描仪(micro CT)检测骨小梁结构,HE染色和抗酒石酸酸性磷酸酶(TRAP)染色观察松质骨形态和破骨细胞成熟情况;采用单因素方差分析(ANOVA)进行组间比较,LSD-t进行事后比较。结果 GD17时铅组孕鼠血铅水平较对照组升高了206%(t=3.876,P=0.003);铅组大鼠骨铅水平始终高于对照组,其密质骨铅含量在整个孕期逐渐升高,而松质骨铅在GD10时较GD3增加了175%(t=4.479,P=0.002),而GD17却较GD10时降低了42.84%(t=-3.017,P=0.015),且GD17时的骨量和骨小梁数量低于对照组,小梁分离度增加,骨组织中成熟破骨细胞数量较对照组显著增多。补充营养素后显著降低了铅暴露所致GD17时升高的血铅水平(52.34%,t=-2.863,P=0.017),并增加血铅排泄率,同时减少松质骨铅排泄率并增加骨铅水平,改善骨微结构;进一步观察发现骨组织中成熟破骨细胞数量也明显减少。结论 孕期钙、VD和Asc的联合补充可显著减轻孕期松质骨铅动员,可能与抑制破骨细胞活化有关。Objective To investigate the effects of calcium,vitamin D(VD),and ascorbic acid(Asc) supplementation on bone lead mobilization during pregnancy.Methods In total 38 newly weaned Wistar female rats were randomly divided into control group(0.05% sodium acetate) and lead exposure group(0.05% lead acetate).They were exposed to distilled water for 4weeks.After 4 weeks of elution,the successfully conceived rats in the lead exposure group were randomly divided into lead group and intervention group.The intervention group was given 0.02% calcium,100 μg/L VD,and 0.2% Asc mixed aqueous solution,and the other groups were given distilled water.Blood and bone samples were collected at 3,10 and 17 days of pregnancy(GD 3,10,and 17).Blood and bone lead levels were measured by inductively coupled plasma mass spectrometry(ICP-MS) and lead excretion rate was calculated.The trabecular structure was detected by microcomputer tomography(micro CT),and cancellous bone morphology and osteoclast maturation were observed by HE staining and tartrate-resistant acid phosphatase(TRAP) staining.Single factor analysis of variance(ANOVA) was used to compare between groups,and LSD-t was used for post hoc comparisons.Results During GD17(t=3.876,P=0.003),the blood lead level of pregnant rats in the lead group was 206% higher than that in the control group.The level of bone lead in the lead group was always higher than that in the control group,and the lead content in dense bone increased gradually during the whole pregnancy.The lead in cancellous bone increased by 175% in GD10 compared with that in GD3(t=4.479,P=0.002),while that in GD17 decreased by 42.84% compared with that in GD10(t=-3.017,P=0.015).The bone mass and the number of trabeculae in GD17 were lower than those in the control group,the trabecular separation increased,and the number of mature osteoclasts in bone tissue increased significantly compared with the control group.Nutrient supplementation significantly reduced the increased blood lead level during lead exposure during GD17(52.34

关 键 词：铅 孕期 骨铅动员 营养素 破骨细胞 

分 类 号：R173[医药卫生—妇幼卫生保健] R715.3[医药卫生—公共卫生与预防医学]