不同温度和外源物质处理对耳叶牛皮消种子萌发的影响  

Effects of Different Temperature and Exogenous Substances on Seed Germination of Cynanchum auriculatum

作　　者：刘方方 黄进婵 段彤 沈敏 洪键 孙淼 张言周 吴承东[3] 康贻军 LIU Fangfang;HUANG Jinchan;DUAN Tong;SHEN Min;HONG Jian;SUN Miao;ZHANG Yanzhou;WU Chengdong;KANG Yijun(School of Marine and Biological Engineering,Yancheng Bioengineering Research Center for Polygonum multiflorum in Binhai,Yancheng Normal University,Yancheng Jiangsu 224007,China;Jiangsu Key Laboratory for Bioresources of Saline Soils,Yancheng Jiangsu 224007,China;Xinyang Agricultural Experiment Station of Yancheng City,Yancheng Jiangsu 224049,China)

机构地区：[1]盐城师范学院海洋与生物工程学院,盐城市滨海白首乌生物工程技术研究中心,江苏盐城224007 [2]江苏省盐土生物资源研究重点实验室,江苏盐城224007 [3]盐城市新洋农业试验站,江苏盐城224049

出　　处：《种子》2023年第6期92-98,共7页Seed

基　　金：国家自然科学基金项目(41977112);江苏省高等学校自然科学研究基金项目(18KJD180008);江苏省盐土生物资源研究重点实验室开放课题项目(72071666003G)。

摘　　要：为探索耳叶牛皮消种子萌发的适宜条件,以种子发芽率、发芽势、发芽指数和发芽进程等为评价依据,考察不同温度、不同种类和浓度的外源物质(植物激素和化学试剂)处理对耳叶牛皮消种子萌发的影响。结果表明,温度对耳叶牛皮消种子萌发有显著影响,25℃培养条件下种子萌发效果最佳,35℃浸种预处理能进一步促进种子萌发;相较于吲哚乙酸(IAA)和6-苄氨基嘌呤(6-BA),外源赤霉素(GA_(3))处理对种子萌发有更显著的促进作用,其中100 mg/L的GA_(3)处理效果最佳,种子发芽率、发芽势和发芽指数均显著高于其他处理组;6 g/L的KNO3和1 g/L的NaCl也能显著促进耳叶牛皮消种子萌发;100 mg/L GA_(3)+6 g/L KNO_(3)+1 g/L NaCl组合处理下,耳叶牛皮消种子发芽率、发芽势和发芽指数分别较对照提高了35.2%、57.6%和94.6%,萌发起始时间和萌发高峰期均较对照提前了2 d,发芽周期也明显缩短。In order to explore the suitable conditions for seed germination of Cynanchum auriculatum,the effects of different temperature,different kinds and concentrations of exogenous substances(plant hormones and chemical reagents)on seed germination were investigated based on germination percentage,germination potential,germination index and germination process.The results showed that temperature had a significant effect on seed germination,and the best germination effect was achieved when cultured at 25℃,while soaking at 35℃ pretreatment could further promote seed germination.Compared with indoleacetic acid(IAA)and 6-benzylaminopurine(6-BA),exogenous gibberellin(GA_(3))had a more significant promoting effect on seed germination,and 100 mg/L GA_(3) treatment had the best effect,where the seed germination percentage,germination potential and germination index were significantly higher than that of other treatment groups.Seed germination of Cynanchum auriculatum was also promoted significantly by KNO_(3)(6 g/L)and NaCl(1 g/L).Under the combined treatment(100 mg/L GA_(3)+6 g/L KNO_(3)+1 g/L NaCl),the germination percentage,germination potential and germination index of Cynanchum auriculatum seeds increased by 35.2%,57.6% and 94.6%,respectively,compared with the control,furthermore,the germination start time and peaking time were both 2 days earlier than control,and the germination cycle was also distinctly shortened.

关 键 词：耳叶牛皮消 种子萌发 温度 激素 

分 类 号：S567.239[农业科学—中草药栽培]