江浙地区菱品种遗传多样性的SLAF-seq分析  

Genetic diversity analysis of Trapa L.cultivars in Jiangsu and Zhejiang Provinces using SLAF-seq

作　　者：袁晔 刘睿[2] 王凌云 沈盟 叶雪莲 权新华 王瑞森 姚祥坦[1] YUAN Ye;LIU Rui;WANG Lingyun;SHEN Meng;YE Xuelian;QUAN Xinhua;WANG Ruisen;YAO Xiangtan(Jiaxing Academy of Agricultural Sciences,Jiaxing 314016,Zhejiang,China;Institute of Vegetable Science,Zhejiang University,Hangzhou 310058,China;Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation,Jinhua Academy of Agricultural Sciences/Zhejiang Institute of Agricultural Machinery,Jinhua 321000,Zhejiang,China)

机构地区：[1]嘉兴市农业科学研究院,浙江嘉兴314016 [2]浙江大学蔬菜研究所,浙江杭州310058 [3]金华市农业科学研究院(浙江省农业机械研究院)浙江省特色水生蔬菜育种与栽培重点实验室,浙江金华321000

出　　处：《浙江农业学报》2023年第8期1773-1781,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省农业(蔬菜)新品种选育重大科技专项(2016C02051-8-1);嘉兴市科技计划项目(2021AZ10013)。

摘　　要：利用SLAF-seq技术对江浙地区17个主要的栽培菱品种和1个野生菱种质进行高通量测序,获得445594个SLAF标签,鉴定到多态性SLAF标签95931个。通过序列分析,获得269338个SNP标记,并基于这些SNP构建18份菱样品的遗传发育树及进行群体结构分析。结果表明,SLAF-seq技术能高效地开发出大量可用于群体遗传分析的SNP标记,基于SNP标记构建的进化树能较好地区分不同类型的菱品种。该结果对开展菱种质资源鉴定和菱种质遗传演化研究具有重要参考价值。By using SLAF-seq technology,17 main cultivated varieties and one wild variety of Trapa L.in Jiangsu and Zhejiang areas were high-throughput sequenced,445594 SLAF tags were obtained,and 95931 polymorphic SLAF tags were identified.A total of 269338 SNP markers obtained were used to construct the phylogenetic tree and analyze the population structure for the 18 samples of Trapa L.The results showed that SLAF-seq technology could efficiently develop a large number of SNP markers which could be used for population genetic analysis.Different types of Trapa L.were distinguished well by the SNP-based phylogenetic analysis.These results provided a reference for the further identification of germplasm resources and the research on the genetic evolution of Trapa L.

关 键 词：菱 遗传多样性 特异性位点扩增片段测序 单核苷酸多态性 

分 类 号：S645.4[农业科学—蔬菜学]