茶园广翅蜡蝉数量与茶叶中生化物质关系分析  

Analysis of relationship between number of Ricanidae in tea plantations and biochemical substances in tea leaves

作　　者：徐悦 吴筱萌 王国庆 邹运鼎[2] 毕守东[1] XU Yue;WU Xiaomeng;WANG Guoqing;ZOU Yunding;BI Shoudong(School of Science,Anhui Agricultural University,Hefei 230036,China;School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学理学院,安徽合肥230036 [2]安徽农业大学林学与园林学院,安徽合肥230036

出　　处：《浙江农业学报》2023年第8期1834-1843,共10页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(30871444);安徽省高校自然科学基金重点项目(KJ2020A0111)。

摘　　要：为明确茶园广翅蜡蝉种群数量与茶叶中生化物质含量的相关性,以期为生物防治和抗虫育种提供科学依据。利用高效液相色谱蒸发光散射检测器(HPLC-ELSD)法测定茶叶中糖类、氨基酸类、儿茶素类、低聚原花青素类、酚酸类、黄酮醇糖苷类这6类离子化合物的含量,运用相关分析方法、灰色关联度分析法、通径分析和回归分析方法研究广翅蜡蝉数量与6类生化物质的关系,得出不同生化物质含量与广翅蜡蝉数量关系的密切程度。由相关系数得出,47种离子化合物中,与广翅蜡蝉的数量强相关性组分为天冬氨酸(-0.6563)、瓜氨酸(-0.6979)、脱落酸(0.6941)、鸟氨基酸(-0.7283)、3-咖啡酰奎宁酸(0.6548)、黄酮醇糖苷K3(0.7414);6大类离子化合物总含量与广翅蜡蝉数量关系为:与广翅蜡蝉数量呈显著相关的为酚酸类和黄酮醇糖苷类;由灰色关联度得出,与广翅蜡蝉数量关系密切的前3位离子组分为3-咖啡酰奎宁酸(0.8497)、黄酮醇糖苷K3(0.8211)、鸟氨基酸(0.7562);由通径系数和回归分析得出,氨基酸类与黄酮醇糖苷类是与广翅蜡蝉数量相关性最显著的2类生化物质。综合分析得出,与广翅蜡蝉数量关系密切的前3位离子组分为3-咖啡酰奎宁酸、黄酮醇糖苷K3和鸟氨基酸;酚酸类和黄酮醇糖苷类为与广翅蜡蝉数量关系较为密切的2类生化物质。该结果可以为茶树抗广翅蜡蝉育种提供参考。To clarify the correlation between the population size of Ricanidae and the content of biochemical substances in tea leaves,to provide the scientific basis for biological control and insect resistance breeding.Contents of 6 types of ionic compounds including sugars,amino acids,catechins,oligomeric proanthocyanidins,phenolic acids and flavonol glycosides in tea leaves were detected by high performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD).The relationship between the number of Ricanidae and 6 types of biochemical substances was studied by using correlation analysis,gray correlation analysis,path analysis and regression analysis,and the closeness of the relationship between the content of different biochemical substances and the number of Ricanidae was obtained.From the correlation coefficients,the strongly correlated components of the 47 ionic compounds with the number of Ricanidae were:aspartic acid(-0.6563),citrulline(-0.6979),abscisic acid(0.6941),ornithine(-0.7283),3-caffeoylquinic acid(0.6548),and flavonol glycoside K3(0.7414).The relationship between the total content of 6 major ionic compounds and the number of Ricanidae was as follows:phenolic acids and flavonol glycosides were significantly correlated with the number of Ricanidae.The top 3 ionic components that were closely related to the number of Ricanidae from the gray correlation were:3-caffeoylquinic acid(0.8497),flavonol glycoside K3(0.8211),and ornithine amino acids(0.7562).From the path coefficients and regression analysis,it was concluded that phenolic acids and flavonol glycosides were the 2 biochemicals with the most significant correlation with the number of Ricanidae.Ionic components closely related to the number of Ricanidae were:3-caffeoylquinic acid,flavonol glycoside K3 and ornithine amino acids.Phenolic acids and flavonol glycosides were two types of biochemical substances closely related to the number of Ricanidae.The results could provide references for breeding tea trees against Ricanidae.

关 键 词：广翅蜡蝉 茶树 生化物质含量 相关系数 灰色关联度 通径系数 回归分析 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]