绿肥种植对红壤旱地生土细菌群落结构的影响  

Response of bacterial community to planting cover crops in virgin upland red soil

作　　者：斯林林 徐静[1] 曹凯[1] 张贤[1] 王建红[1] SI Linlin;XU Jing;CAO Kai;ZHANG Xian;WANG Jianhong(Institute of Environment and Resource&Soil Fertilizer,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院环境资源与土壤肥料研究所,浙江杭州310021

出　　处：《浙江农业学报》2023年第8期1864-1875,共12页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(420107358);国家绿肥产业技术体系(CARS-22);浙江省自然科学基金(LQ20C030005)。

摘　　要：为探究红壤旱地生土种植绿肥对土壤细菌群落结构的影响,在浙江省遂昌县和景宁畲族自治县的新垦旱地设置不种植绿肥的对照,以及种植大绿豆和种植高丹草的处理,利用高通量测序技术研究不同处理表层土壤细菌多样性和群落结构的差异,并分析细菌群落与环境因子的相关性。结果表明,与对照相比,种植绿肥显著(P<0.05)提高了土壤微生物生物量碳、氮含量,且高丹草的效果优于大绿豆。种植两种绿肥作物对细菌群落多样性无显著影响。不同处理下的优势细菌门类为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、放线菌门(Actinobacteriota)、酸杆菌门(Acidobacteriota)、浮霉菌门(Planctomycetota)、黏球菌门(Myxococcota)、疣微菌门(Verrucomicrobiota),相对丰度共占84%以上。与对照相比,种植绿肥降低了差异标志物的数量,尤其是种植大绿豆的处理。在门水平上,种植大绿豆较高丹草大幅提高了根际土壤中拟杆菌门(Bacteroidota)的相对丰度;在属水平上,种植大绿豆处理的罗河杆菌属(Rhodanobacter)和孤岛杆菌属(Dokdonella)的相对丰度较种植高丹草的显著(P<0.05)提高5~6倍。冗余分析和相关分析发现,影响土壤细菌髌骨细菌门(Patescibacteria)、拟杆菌门、放线菌门、芽单孢菌门(Gemmatimonadota)、厚壁菌门(Firmicutes)、黏球菌门、绿弯菌门和变形菌门分布的主要环境因子是pH值、速效钾和微生物生物量碳含量。综上,在红壤旱地生土上种植绿肥作物有助于改善土壤环境条件,促进土壤细菌群落参与土壤养分循环。In the present assay,field experiments were conducted to investigate the effect of 3 treatments,i.e.,non-planting(CK),planting bean(Phaseolus vulgaris L.var.humilis Alef),and planting sorghum-sudangrass hybrid(Sorghum bicolor×S.sudanense)on the bacterial community structure of virgin red soils under newly-cultivated upland,in Suichang County and Jingning She Autonomous County,Zhejiang Province,China,respectively.High-throughput sequencing technology was used to study the diversity and community structure of bacteria in soil.Moreover,the correlation within soil environmental factors and bacterial communities was analyzed.The results showed that significant(P<0.05)differences were observed in the soil microbial biomass carbon and nitrogen content after planting cover crops,and the effect of sorghum-sudangrass hybrid was better than that of bean.The diversity of bacterial communities did not alter significantly between the 2 types of cover crops.The predominant phyla were Proteobacteria,Chloroflexi,Actinobacteriota,Acidobacteriota,Planctomycetota,Myxococcota,and Verrucomicrobiota,of which the relative abundance accounted for over 84%.Planting cover crops decreased the qualities of distinct biomarkers,especially when planting beans.At the phylum level,beans dramatically increased the relative abundance of Bacteroidota in rhizosphere soils compared with the sorghum-sudangrass hybrid.Moreover,the relative abundance of Rhodanobacter and Dokdonella in bean rhizosphere soils was significantly(P<0.05)increased by 5-6 folds compared with the sorghum-sudangrass hybrid under the genus level.Redundancy analysis and correlation analysis revealed that pH,available potassium content,and soil microbial biomass carbon content were the main environmental factors affecting the distribution of soil bacterial phyla,such as Patescibacteria,Bacteroidota,Actinobacteriota,Gemmatimonadota,Firmicutes,Myxococcota,Chloroflexi,Proteobacteria.Overall,planting cover crops on virgin upland red soils could improve soil environmental conditions and

关 键 词：绿肥 红壤 旱地 生土 土壤细菌 群落结构 

分 类 号：S142[农业科学—肥料学]