蒙古族肥胖儿童肠道菌群结构和短链脂肪酸含量的研究  

ANALYSIS OF INTESTINAL FLORA STRUCTUREAND SHORT-CHAIN FATTY ACID CONTENT IN OBESE MONGOLIAN CHILDREN

作　　者：商佳琪 青格乐 郭宇帆 田春风 包艳[1,2] SHANG Jia-qi;QING Ge-le;GUO Yu-fan;TIAN Chun-feng;BAO Yan(School of Public Health,Baotou Medical College,Baotou O10460;Institute of Nutrition and Food Health,Baotou Medical College,Baotou 010460;Damaoqi Center for Disease Control and Prevention,Baotou Damao Banner014500;Ordos Center for Disease Control and Prevention,Ordos017010,China)

机构地区：[1]包头医学院公共卫生学院,包头014060 [2]包头医学院营养与食品健康研究所,包头014060 [3]达茂旗疾病预防控制中心,包头达茂旗014500 [4]鄂尔多斯市疾病预防控制中心,鄂尔多斯017010

出　　处：《营养学报》2023年第3期259-263,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81560150);内蒙古自治区自然科学基金[No.2017MS(LH)0815];包头医学院花蕾计划(大学生创新训练)项目(No.110-2019311313);包头医学院“问学计划”、“为学计划”、“践学计划”项目(No.2019BYWWJ-YB-02);内蒙古自治区全区研究生教育教学改革研究与实践项目[No.YJG201910127(Y02)]。

摘　　要：目的 了解蒙古族肥胖和正常体重儿童肠道菌群结构及其代谢产物短链脂肪酸的含量,为蒙古族肥胖的研究提供一定基础数据。方法 采用随机整群抽样的方法按照性别年龄1:1配比,正常体重组和肥胖组各30人,取新鲜粪便提取肠道菌群总DNA,进行总DNA Illumina Miseq测序;用SYBR嵌合荧光法进行实时荧光定量PCR;通过气相色谱法测定各粪便样本中SCFAs的含量。结果 蒙古族肥胖儿童肠道菌群的多样性和丰富度均低于正常体重组(P<0.05);门水平以Bacteroidetes(正常组56.49%,肥胖组47.08%)和Firmicutes(正常组38.61%,肥胖组48.73%)的相对丰度最高;两组的共同优势菌属有17种,在优势菌属中Pseudobutyrivibrio、 Parasutterella、Ruminococcaceae_UCG_014、Bifidobacterium在两组间的差异明显(P<0.05);SCFAs的含量在正常体重组与肥胖组间差异明显(P<0.05)。结论 蒙古族肥胖儿童肠道菌群的结构及短链脂肪酸含量均发生改变,揭示肠道菌群结构变化对于蒙古族儿童肥胖的发生可能起到一定的作用。l Objective To investigate the structural composition of the intestinal flora and the content of short-chain fatty acids Mongolian obese and normal-weight children,so as to provide basic data for the study of Mongolian obesity.Methods A random cluster sampling method was used to recruit 30 normal and 30 obese children with half male and half female.Fresh feces were taken to extract the total DNA of the intestinal flora,and the total DNA was sequenced by Ilumina Miseq method.SYBR chimeric real-time fluorescent quantitative PCR was performed.The content of SCFAs in each stool sample was determined by gas chromatography.Results The diversity and richness of the intestinal flora of the Mongolian obese children were lower than those of the normal children(P<0.05).At the phylum level,Bacteroidetes were 56.49%in the normal group,47.08%in the obese group while Firmicutes were 38.61%in the normal group,and 48.73%in the obesity group.There were 17 common dominant bacteria in the two groups.Among the dominant bacteria,Pseudobutyrivibrio,Parasutterella,Ruminococcaceae_UCG_014,and Bifidobacterium were significantly different between the two groups(P<0.05);In the content of SCFAs,there was a significant difference between the two groups(P<0.05).Conclusion The structure of the intestinal flora and the content of SCFAs in obese Mongolian children are changed,and may play a certain role in the occurrence of obesity in Mongolian children.

关 键 词：肠道菌群 短链脂肪酸 肥胖 蒙古族儿童 

分 类 号：R179[医药卫生—妇幼卫生保健] R723.14[医药卫生—公共卫生与预防医学]