近红外光激发氧化石墨烯/磷酸银/壳聚糖涂层改善钛表面抗菌性能的研究  

Near-infrared excited graphene oxide/silver nitrate/chitosan coating for improving antibacterial properties of titanium implants

作　　者：王轶凡 许英德 张雪枫 刘菁宇 韩金彤 朱胜利[1] 梁砚琴[1] 吴水林[1] 崔振铎[1] 吕维加[2] 李朝阳[1] WANG Yifan;XU Yingde;ZHANG Xuefeng;LIU Jingyu;HAN Jintong;ZHU Shengli;LIANG Yanqin;WU Shuilin;CUI Zhenduo;LÜWeijia;LI Zhaoyang(Tianjin Key Laboratory of Composite and Functional Materials,School of Materials Science and Engineering,Tianjin University,Tianjin,300350,P.R.China;Faculty of Pharmaceutical Sciences,Shenzhen Institute of Advanced Technology Chinese Academy of Sciences,Shenzhen Guangdong,518055,P.R.China)

机构地区：[1]天津市复合与功能材料重点实验室、天津大学材料科学与工程学院,天津300350 [2]中国科学院深圳先进技术研究院药学院,广东深圳518055

出　　处：《中国修复重建外科杂志》2023年第8期937-944,共8页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(51871163);天津市自然科学基金资助项目(20JCZDJC00580)。

摘　　要：目的设计并构建一种氧化石墨烯(graphene oxide,GO)/磷酸银(Ag_(3)PO_(4))/壳聚糖(chitosan,CS)复合涂层,用于植入物手术中快速杀灭细菌,预防术后感染。方法使用离子交换法制备GO/Ag_(3)PO_(4)复合材料,并在生物钛(Ti)片上依次沉积CS和GO/Ag_(3)PO_(4)复合材料,制备Ti-GO/Ag_(3)PO_(4)/CS材料。对材料的形貌、物相、光热和光催化性能、抗菌能力以及与基体的结合力进行表征。结果使用离子交换法成功制备GO/Ag_(3)PO_(4)复合材料,通过形貌物相测试证明了GO/Ag_(3)PO_(4)的异质结构,Ag_(3)PO_(4)和GO形成的异质结构使带隙从1.79 eV降低到1.39 eV,可以被808 nm近红外光激发。光热、光催化实验表明GO/Ag_(3)PO_(4)/CS复合涂层具有良好的光热和光动力性能;体外抗菌实验表明,GO/Ag_(3)PO_(4)/CS复合涂层在808 nm近红外光照射20 min后对金黄色葡萄球菌的抑菌率达到99.81%;同时该复合涂层具有良好的光稳定性,可以提供稳定和持续的抗菌效果。结论GO/Ag_(3)PO_(4)/CS复合涂层可以被808 nm近红外光激发产生活性氧,在光照下具有优异的抗菌能力。Objective To design and construct a graphene oxide(GO)/silver nitrate(Ag_(3)PO_(4))/chitosan(CS)composite coating for rapidly killing bacteria and preventing postoperative infection in implant surgery.Methods GO/Ag_(3)PO_(4) composites were prepared by ion exchange method,and CS and GO/Ag_(3)PO_(4) composites were deposited on medical titanium(Ti)sheets successively.The morphology,physical image,photothermal and photocatalytic ability,antibacterial ability,and adhesion to the matrix of the materials were characterized.Results The GO/Ag_(3)PO_(4) composites were successfully prepared by ion exchange method and the heterogeneous structure of GO/Ag_(3)PO_(4) was proved by morphology phase test.The heterogeneous structure formed by Ag_(3)PO_(4) and GO reduced the band gap from 1.79 eV to 1.39 eV which could be excited by 808 nm near-infrared light.The photothermal and photocatalytic experiments proved that the GO/Ag_(3)PO_(4)/CS coating had excellent photothermal and photodynamic properties.In vitro antibacterial experiments showed that the antibacterial rate of the GO/Ag_(3)PO_(4)/CS composite coating against Staphylococcus aureus reached 99.81%after 20 minutes irradiation with 808 nm near-infrared light.At the same time,the composite coating had excellent light stability,which could provide stable and sustained antibacterial effect.Conclusion GO/Ag_(3)PO_(4)/CS coating can be excited by 808 nm near infrared light to produce reactive oxygen species,which has excellent antibacterial activity under light.

关 键 词：细菌感染 抗菌涂层 异质结构 光热-光动力抗菌 

分 类 号：R318.08[医药卫生—生物医学工程]