化瘀散指纹图谱的建立与化学模式识别、有效成分含量测定  

Establishment of fingerprint,chemical pattern recognition analysis and main component determination of Huayu powder

作　　者：邱昭昭 刘心怡 赵旻[1] 王淼[3] 赵春杰[1] QIU Zhaozhao;LIU Xinyi;ZHAO Min;WANG Miao;ZHAO Chunjie(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Business Administration,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学工商管理学院,辽宁沈阳110016 [3]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2023年第7期882-889,934,共9页Journal of Shenyang Pharmaceutical University

摘　　要：目的结合化学模式识别,分析化瘀散的HPLC指纹图谱,并测定其5种有效成分的含量。方法以双去甲氧基姜黄素为参照,绘制10批化瘀散的HPLC指纹图谱,进行相似度评价,确定共有峰;运用统计学软件进行聚类分析、主成分分析和正交偏最小二乘法分析;同时测定化瘀散中5种有效成分的含量。结果10批化瘀散标定16个共有峰,相似度大于0.98;指认了人参皂苷Rg1、人参皂苷Rb1、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素5个共有峰;聚类分析显示10批样品可聚为3类,PCA显示3个主成分的累积方差贡献率为89.914%,OPLS-DA显示峰9、15、2、16可作为鉴别和区分化瘀散质量的标志物;5个峰的线性相关系数r>0.9995,含量测定结果范围分别为3.802~3.931、2.889~3.018、3.389~3.435、4.122~4.191、6.579~6.728 mg·g^(-1)。结论基于化学模式识别的指纹图谱分析和含量测定方法,可用于化瘀散的质量控制。Objective Box-Behnken response surface design method combined with entropy weight method was used to optimize the optimal extraction process of volatile oil from Angelica Sinensis and Cassia Twig in Danggui Sini decoction.Methods The liquid-material ratio,soaking time and the proportion of sodium chloride solution were used as the investigation factors,and the contents of two fat soluble components(ligustilide and cinnamaldehyde)in volatile oil were taken as the investigation indexes.Based on the single factor experiment,the optimal range of factors was optimized.The Box-Behnken response surface design method was used to construct the three factor and three-level experimental design scheme,and the entropy weight method was used to weight the two indexes.The comprehensive score results were used as the evaluation indexes of the extraction process.The optimal process conditions were optimized,and the process was verified.Results The optimal technological conditions of volatile oil from Angelica Sinensis and Cassia Twig were as follows:liquid material ratio was 4.6∶1,soaking time was 1.14 h,sodium chloride addition ratio was 5.7∶1,extraction time was 4 h,extracted once,and the crushing degree of medicinal materials was all through No.1 sieve.Conclusion Box-Behnken response surface design method is stable and has good predictability,which can be used to optimize the volatile oil extraction process of Danggui Sini decoction.It has great guiding significance for the study of volatile oil extraction process of Danggui Sini decoction.

关 键 词：化瘀散 指纹图谱 高效液相色谱法 含量测定 化学模式识别 

分 类 号：R917[医药卫生—药物分析学]