近红外比率荧光探针双重响应SO_(2)衍生物和ONOO^(-)  

A Near-infrared Ratiometric Fluorescent Probe for Dual-response of SO_(2) Derivatives and ONOO^(-)

作　　者：马素芳 耿佳美 刁海鹏[1] 郭丽霞 刘文[1] 张承武 MA Sufang;GENG Jiamei;DIAO Haipeng;GUO Lixia;LIU Wen;ZHANG Chengwu(School of Basic Medical Science,Shanxi Medical University,Taiyuan 030001,China;School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学药学院,山西太原030001

出　　处：《山西大学学报（自然科学版）》2023年第4期888-896,共9页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(82202289,82071969);山西省青年科技研究基金(20210302124341);山西省自然科学基金(201901D111191,20210302123294,20210302123299)。

摘　　要：同时检测生物体内二氧化硫衍生物(HSO_(3)^(-)/SO_(3)^(2-))和过氧亚硝酸根(ONOO^(-))对深入研究相关疾病的发生和发展具有重要意义。本文合成了一种双响应比率荧光探针(NI),用于HSO_(3)^(-)/SO_(3)^(2-)和ONOO^(-)的双重检测。在该探针中,半花菁被选为荧光发色团,碳碳双键被作为识别单元。NI发射的近红外荧光(746 nm)可分别切换至绿光(582 nm)和蓝光(406 nm),以分别响应HSO_(3)^(-)/SO_(3)^(2-)和ONOO^(-)。研究结果表明,该探针具有较强的特异性和较高的灵敏度,可以用于肝癌细胞HepG2中HSO_(3)^(-)/SO_(3)^(2-)和ONOO^(-)的双重检测。The simultaneous detection of sulfur dioxide derivatives(HSO_(3)^(-)/SO_(3)^(2-))and peroxynitrite(ONOO^(-))in vivo is immensely significant for deeply investigating the occurrence and progress of related diseases.A dual-responsive ratiometric fluorescent probe(NI)for the simultaneous detection of HSO_(3)^(-)/SO_(3)^(2-)and ONOO^(-)was synthesized.In this probe,semi-cyanine was chosen as the chromophore and carbon-carbon double bond was chosen as the recognition unit.The probe NI emitted near-infrared fluorescence(746 nm),which can switch to green fluorescence(582 nm)and blue fluorescence(406 nm)in response to HSO_(3)^(-)/SO_(3)^(2-)and ONOO^(-),respectively.The results demonstrated that the probe exhibit high specificity and sensitivity,and can be used for the detection of HSO_(3)^(-)/SO_(3)^(2-)and ONOO^(-)in HepG2 cells.

关 键 词：荧光探针 二氧化硫衍生物 过氧亚硝酸根 荧光成像 

分 类 号：O657.3[理学—分析化学]