濒危药用植物大花杓兰地下芽的组织培养  

Tissue Culture of Underground Buds of the Endangered Medicinal Plant Cypripedium macranthos Sw

作　　者：郭鹏媛 李冲冲 周悦 樊杰 GUO Pengyuan;LI Chongchong;ZHOU Yue;FAN Jie(School of Chinese Medicine and Food Engineering,Shanxi University of Chinese Medicine,Jinzhong,Shanxi 030619,China)

机构地区：[1]山西中医药大学中药与食品工程学院,山西晋中030619

出　　处：《热带农业科学》2023年第7期31-36,共6页Chinese Journal of Tropical Agriculture

基　　金：山西省中医药管理局项目(No.2020ZYYC002);山西中医药大学青苗项目(No.2018PY-017)。

摘　　要：为有效提高大花杓兰地下芽的成活率,优化其生长条件,以大花杓兰花期、果期和休眠期的地下芽为材料,在单因素试验的基础上,通过L9(34)正交试验对组织培养方法进行优化,探索培养基、6-BA、NAA和pH对大花杓兰地下芽成活率的影响。结果表明:休眠期微剥鳞叶芽成活率优于花期和果期的成活率,最高达84.44%;地下芽成活率影响因素主次顺序为培养基>6-BA>NAA>pH;大花杓兰地下芽的最佳培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA+pH 6,其成活率高达73.33%,且地下芽生长状态良好,苗株健康。优化后的组织培养方法能显著提高大花杓兰地下芽成活率,为大花杓兰苗株大规模培养提供理论参考。To effectively improve the survival rate and growth conditions of the underground buds of Cypripedium macranthos Sw,the tissue culture method was optimized by L9(34)orthogonal test based on a single-factor experiment,and the effects of medium,6-BA,NAA,and pH on the survival rate of underground buds of C.macranthos Sw were explored.The results showed that the survival rate of micro-scaly leaf buds in the dormant stage was better than that at the flowering and fruit stages,up to 84.44%.The primary and secondary factors influencing the survival rate of underground buds were the medium>6-BA>NAA>pH.The best medium for underground buds of C.macranthos Sw was MS+1.0 mg·L^(–1)6-BA+0.1 mg·L^(–1)NAA+pH 6,and the survival rate was as high as 73.33%,the underground buds were in good condition and the seedlings were healthy.The optimized tissue culture method can significantly improve its survival rate,which provides a theoretical reference for the large-scale cultivation of plants of C.macranthos Sw.

关 键 词：大花杓兰 地下芽 组织培养 单因素试验 正交设计 

分 类 号：S682.31[农业科学—观赏园艺]