三七皂苷R1对紫外线照射人成纤维细胞急性光损伤的保护作用  

Protective Effect of Notoginsenoside R1 on Acute Photo-Damage of Human Fibroblasts Induced by UVA

作　　者：毛静 王亚琳 安全 包丽娟 李旺 MAO Jing;WANG Yalin;AN Quan;BAO Lijuan;LI Wang(Yunnan Baiyao Group Co.,Ltd.,Kunming 650500,Yunnan,China)

机构地区：[1]云南白药集团股份有限公司,云南昆明650500

出　　处：《香料香精化妆品》2023年第4期46-50,共5页Flavour Fragrance Cosmetics

摘　　要：研究了三七皂苷R1对长波紫外线(UVA)照射人成纤维细胞(human fibroblast,HFB)所导致的急性光损伤的保护作用。采用噻唑蓝(MTT)细胞活性法检测样品在HFB上的最大安全给药剂量,通过将HFB分为UVA组、UVA+三七皂苷R1组(50、100、200μg/mL)和空白组(不予UVA照射),开展RNA提取、反转录及荧光定量PCR操作,评价HFB细胞外基质(ECM)合成相关基因CollagenⅠ、CollagenⅢ、CollagenⅦ、Elastin和ECM降解相关基因基质金属蛋白酶-3(MMP-3)的表达水平。结果表明,5 J/cm2的UVA照射可显著抑制CollagenⅠ、CollagenⅢ和Elastin基因的表达,并显著提升MMP-3的表达水平。而50μg/mL三七皂苷R1可在5 J/cm2的UVA刺激条件下,显著提升CollagenⅠ、CollagenⅢ、CollagenⅦ和Elastin的表达水平,从而抑制因UVA照射导致的HFB急性光损伤。The protective effect of notoginsenoside R1 on acute photodamage caused by long-wave ultraviolet(UVA)irradiation of human fibroblasts(HFB)was investigated.Methyl thiazolyl tetrazolium(MTT)was used to determine the maximum safe dose of the samples on HFB.Then human fibroblasts were divided into UVA group,UVA combined with notoginsenoside R1 group(50,100,200μg/mL)and blank control group(without UVA).RNA extraction,reverse transcription and fluorescence quantitative PCR operations were conducted to evaluate the expression levels of HFB extracellular matrix(ECM)-synthesis-related genes CollagenⅠ,CollagenⅢ,CollagenⅦ,Elastin and ECM degradation-related gene matrix metalloproteinase-3(MMP-3).The results showed that UVA irradiation at 5 J/cm2 significantly inhibited the expression of CollagenⅠ,CollagenⅢand Elastin genes,and significantly enhance the expression of MMP-3.However,50μg/mL notoginsenoside R1 significantly elevated the expression of CollagenⅠ,CollagenⅢ,CollagenⅦand Elastin under UVA irradiation at 5 J/cm2,thus inhibiting the acute photodamage of HFB induced by UVA irradiation.

关 键 词：三七 三七皂苷 R1 人成纤维细胞 皮肤光老化 

分 类 号：R285.5[医药卫生—中药学]