蜂毒素-死亡素重组抗菌肽的小鼠毒性试验  

Toxicity Test of Melittin-Thanatin Recombinant Antimicrobial Peptide in Mice

作　　者：刘小元 孟春颜 姬生跃 刘勇[3] LIU Xiaoyuan;MENG Chunyan;JI Shengyue;LIU Yong(Xiuwu County Agricultural Comprehensive Administrative Law Enforcement Brigade,Xiuwu Henan 454351,China;Zhongji Hi-Tech(Beijing)Biotechnology Co.,Ltd.;College of Veterinary Medicine,Northwest Agriculture and Forestry University)

机构地区：[1]修武县农业综合行政执法大队,河南修武454351 [2]中基高科(北京)生物技术有限公司 [3]西北农林科技大学

出　　处：《畜牧兽医杂志》2023年第5期25-28,共4页Journal of Animal Science and Veterinary Medicine

基　　金：陕西省动物防疫专项资金项目(2021-XN18、XNDY2201);宁夏回族自治区重点研发计划(2021BEF03005)。

摘　　要：本试验旨在探索蜂毒素-死亡素重组抗菌肽(MT-W)对昆明小鼠的毒性影响。试验通过小鼠口服急性毒性试验和口服生物利用度试验,研究了MT-W对小鼠主要脏器的影响,并检测了血液中碱性磷酸酶、丙氨酸氨基转移酶、天冬氨酸氨基转移酶等生化指标,并对MT-W的体内吸收情况进行了检测。结果显示,MT-W在试验设定浓度下,对试验小鼠的体重、器官/体重比率没有显著影响,血液生化指标与对照组也无统计学差异;在按照50 mg/kg体重给实验小鼠灌服MT-W溶液后,在血浆中未检测到MT-W。研究表明,MT-W对小鼠没有明显毒副作用,MT-W在口服后未被有效吸收。The aim of this experiment was to investigate the toxicity effects of melittin-thanatin recombinant antimicrobial peptide(MT-W)on Kunming mice.The effects of MT-W on major organs of mice were studied by oral acute toxicity test and oral bioavailability test,and biochemical indexes such as alkaline phosphatase,alanine aminotransferase and aspartate amin-otransferase in blood were detected,as well as the absorption of MT-W in vivo.The results showed that MT-W had no sig-nificant effects on the body weight and organ/body weight ratio of experimental mice,and there was no statistical difference in blood biochemical indexes between the control group and the control group.No MT-W was detected in plasma of mice given MT-W solution at 50mg/kg body weight.Studies have shown that MT-W has no obvious toxic and side effects on mice,and MT-W is not effectively absorbed after oral administration.

关 键 词：蜂毒素 死亡素 重组抗菌肽 小鼠 毒性试验 

分 类 号：S896.5[农业科学—特种经济动物饲养]