几种化学物质对白纹伊蚊的引诱效果研究  

Attractive action of different chemical substances for Aedes albopictus

作　　者：王飞[1] 徐皓迪 李鑫 冷培恩[2] 刘洪霞[2] WANG Fei;XU Hao-di;LI Xin;LENG Pei-en;LIU Hong-xia(Disinfection and Vector Control Department,Hongkou District Center for Disease Control and Prevention,Shanghai 200082,China;Department of Vector Control,Shanghai Center for Disease Control and Prevention,Shanghai 200336,China)

机构地区：[1]上海市虹口区疾病预防控制中心消毒病媒防制科,上海200082 [2]上海市疾病预防控制中心病媒生物防治科,上海200336

出　　处：《中国媒介生物学及控制杂志》2023年第4期447-450,496,共5页Chinese Journal of Vector Biology and Control

基　　金：上海市卫生健康委员会科研课题(201940372);国家科技重大专项“十三五”课题(2017ZX10303404001006);上海市卫生健康委员会卫生行业临床研究专项(202240331)。

摘　　要：目的通过室内筛选对白纹伊蚊有引诱作用的化学物质,进行现场效果评价,探讨白纹伊蚊对6种人体气味物质的偏好程度。方法在实验室条件下,测试浓度为0.1、1.0、10.0 mg/ml的6种化学物质(1-辛烯-3-醇、乳酸、肉豆蔻酸、氨水、丙酮和乙酸乙酯)单独使用时对白纹伊蚊雌蚊的引诱作用;将上述6种化合物,按照一定浓度,1∶1比例两两混合,测试对白纹伊蚊雌蚊的引诱作用;将乳酸加入到两两混合试验筛选出来效果较好的引诱剂配方,测试对白纹伊蚊雌蚊的引诱作用。将室内筛选出的引诱剂配方应用于现场测试对白纹伊蚊的引诱效果。不同化合物对白纹伊蚊引诱作用的差异采用t检验和单因素方差分析。结果0.1、1.0和10.0 mg/ml的1-辛烯-3-醇,1.0和10.0 mg/ml肉豆蔻酸,0.1 mg/ml乙酸乙酯单独使用时对白纹伊蚊有引诱作用;其他3种物质各浓度对白纹伊蚊雌蚊无明显引诱效果。肉豆蔻酸+1-辛烯-3-醇、乳酸+肉豆蔻酸、乳酸+1-辛烯-3-醇对白纹伊蚊均有引诱作用,与对照组比较诱蚊数量差异均有统计学意义(t=8.102,P=0.001;t=4.696,P=0.009;t=5.127,P=0.007)。乳酸+肉豆蔻酸+1-辛烯-3-醇对白纹伊蚊有明显的引诱性,与对照组比较诱蚊数量差异有统计学意义(F=86.841,P<0.001)。室外试验测试乳酸+肉豆蔻酸+1-辛烯-3-醇对白纹伊蚊的引诱作用,与空白对照组比较诱蚊数量差异有统计学意义(第1天:t=7.462,P=0.014;第2天:t=20.500,P<0.001;第3天:t=9.383,P=0.001)。结论乳酸、肉豆蔻酸和1-辛烯-3-醇的混合物对白纹伊蚊具有一定引诱作用,有必要进一步开展野外研究。Objective To select chemical substances with attractive effects on Aedes albopictus for on-field assessment,and to explore the preference of Ae.albopictus to six human odors.Methods Under laboratory conditions,six chemical substances(1-octen-3-ol,lactic acid,myristic acid,ammonia,acetone,and ethyl acetate)were separately tested for their individual attracting effects on female Ae.albopictus at concentrations of 0.1,1.0,and 10.0 mg/ml.The six substances were mixed in pairs(1∶1)at certain concentrations,and then the pairs were tested for attracting effects on female Ae.albopictus.The pair with good attractive effects was combined with lactic acid for testing.The attractant combination selected by laboratory experiments was tested for attractive action for Ae.albopictus in the field.The attracting effects of the substances or combinations on Ae.albopictus were compared using the t test and one-way analysis of variance.Results Ae.albopictus mosquitoes were attracted to 1-octen-3-ol at 0.1,1.0,and 10.0 mg/ml,myristic acid at 1.0 and 10.0 mg/ml,and ethyl acetate at 0.1 mg/ml,but not to the other three substances at any concentration.The pairs of 1-octen-3-ol+myristic acid,lactic acid+myristic acid,and lactic acid+1-octen-3-ol statistically attracted Ae.albopictus compared with the control group(t=8.102,P=0.001;t=4.696,P=0.009;t=5.127,P=0.007).The combination of lactic acid+1-octen-3-ol+myristic acid statistically attracted Ae.albopictus compared with the control group(F=86.841,P<0.001).Field testing showed significant differences in the attracting effect between lactic acid+1-octen-3-ol+myristic acid and the control(day 1:t=7.462,P=0.014;day 2:t=20.500,P<0.001;day3:t=9.383,P=0.001).Conclusion The combination of lactic acid,1-octen-3-ol,and myristic acid has attractive action for Ae.albopictus,which should be further researched in the field.

关 键 词：白纹伊蚊 引诱化合物 引诱作用 

分 类 号：R384.1[医药卫生—医学寄生虫学]