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作 者:张晨曦 武少卿 刘洋 刘芳 王宇[5] 范蒙光 李建云 ZHANG Chen-xi;WU Shao-qing;LIU Yang;LIU Fang;WANG Yu;FAN Meng-guang;LI Jian-yun(School of Public Health,Inner Mongolia Medical University,Hohhot,Inner Mongolia 010110,China;Ulanqab Center for Disease Prevention and Control,Ulanqab,Inner Mongolia 012000,China;Baotou Medical College,Baotou,Inner Mongolia 014000,China;Inner Mongolia Center for Comprehensive Disease Prevention and Control,Hohhot,Inner Mongolia 010031,China;Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China)
机构地区:[1]内蒙古医科大学公共卫生学院,内蒙古呼和浩特010110 [2]乌兰察布市疾病预防控制中心,内蒙古乌兰察布012000 [3]包头医学院,内蒙古包头014000 [4]内蒙古自治区综合疾病预防控制中心,内蒙古呼和浩特010031 [5]内蒙古农业大学,内蒙古呼和浩特010018
出 处:《中国媒介生物学及控制杂志》2023年第4期559-563,共5页Chinese Journal of Vector Biology and Control
基 金:内蒙古自治区科技重大专项(2021ZD0006);内蒙古自治区自然科学基金(2018MS08059);国家重点研发计划(2021YFC1200200)。
摘 要:目的了解我国内蒙古自治区(内蒙古)森林革蜱感染立克次体、无形体及埃立克体等病原体情况,并研究其遗传多样性。方法在内蒙古乌兰察布市四子王旗、呼和浩特市土默特左旗家畜体表采集寄生蜱成蜱76只,经形态学和分子生物学鉴定后,用针对16S rRNA基因和柠檬酸合成酶基因(gltA)保守区域的引物同时检测立克次体,用针对16S rRNA基因的属特异性引物检测埃立克体、无形体。对检出的阳性样本,使用巢式PCR扩增获得gltA和热休克蛋白基因(groEL)。用PhyML 3.0软件进行最大似然法进化树构建。结果采获蜱经鉴定均为森林革蜱。从上述采集蜱中检出立克次体阳性8份,其中四子王旗6份(6/35,17.14%),土默特左旗2份(2/41,4.88%),测序结果显示均为劳氏立克次体。对3个基因序列的进化分析,上述菌株分成2个不同的分支,当地流行的劳氏立克次体有一定遗传多样性。未检出埃立克体和无形体。结论内蒙古的革蜱中存在劳氏立克次体的流行,且存在一定遗传多样性。劳氏立克次体为人类病原体,在内蒙古局部地区较高的阳性率提示可能存在感染人的风险。Objective To investigate the situation of Rickettsia,Anaplasma,and Ehrlichia infections in Dermacentor silvarum and their genetic diversity in the Inner Mongolia Autonomous Region(Inner Mongolia),China.Methods A total of 76 adult parasitic ticks were collected from the body surface of livestock in Dorbod Banner in Ulanqab and Tumed Left Banner in Hohhot,Inner Mongolia.All of them were morphologically and molecularly identified.Rickettsia in the ticks was examined using primers targeting the conserved regions of both 16S rRNA and citrate synthase(gltA)genes.Anaplasma and Ehrlichia were examined using genus-specific primers targeting the 16S rRNA gene.For positive specimens,nested PCR was used to amplify the gltA gene and the heat shock protein gene(groEL).PhyML 3.0 software was used for constructing maximum likelihood phylogenetic trees.Results All the captured ticks were identified as D.silvarum.A total of 8 ticks tested positive for Rickettsia,with six(6/35,17.14%)from Dorbod Banner and two(2/41,4.88%)from Tumed Left Banner,all being identified as R.raoultii by sequencing.The phylogenetic analysis based on the 16S rRNA,gltA,and groEL genes revealed that the strains were divided into two clades in the phylogenetic tree,suggesting that R.raoultii was genetically diverse in this area.All the samples were negative for Anaplasma and Ehrlichia.Conclusions R.raoultii is epidemic among Dermacentor ticks in Inner Mongolia,with certain genetic diversity.Concerning its human pathogenicity,the high positive rate of R.raoultii in certain areas of Inner Mongolia suggests a potential risk of human infections.
关 键 词:内蒙古 四子王旗 土默特左旗 森林革蜱 劳氏立克次体
分 类 号:R384.4[医药卫生—医学寄生虫学] R376[医药卫生—基础医学]
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