葱白提取物对油酸诱导HepG2细胞脂代谢的影响及其机制  

Effect and Mechanism of Extracts from Allium cepa var.aggregatum on Lipid Metabolism of HepG2 Cells Induced by Oleic Acid

作　　者：胡松 刘莉[1,2,3] 李璐璐 胡磊 邹冲冲 黄传奇 刘慷洁 孙安妮 HU Song;LIU Li;LI Lulu;HU Lei;ZOU Chongchong;HUANG Chuanqi;LIU Kangjie;SUN Anni(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China;Department of Pharmacy,Wuhan Hospital of Traditional Chinese and Western Medicine,Wuhan 430022,China;Department of Pharmacy,Wuhan Brain Hospital,General Hospital of Yangtze River Shipping,Wuhan 430019,China;Department of Pharmacy,Liyuan Hospital Affilited to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430077,China)

机构地区：[1]湖北中医药大学药学院,武汉430065 [2]武汉市中西医结合医院药学部,武汉430022 [3]长江航运总医院、武汉脑科医院药学部,武汉430019 [4]华中科技大学同济医学院附属梨园医院药剂科,武汉430077

出　　处：《医药导报》2023年第9期1325-1331,共7页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(2013CFB367)。

摘　　要：目的以油酸诱导的HepG2细胞为模型,探讨葱白提取物调节脂代谢的作用及其机制。方法噻唑蓝(MTT)法选择适宜的葱白提取物(ACAE)实验浓度后,酶联免疫吸附测定(ELISA)法检测不同浓度ACAE对油酸诱导的HepG2细胞三酰甘油(TG)、活性氧(ROS)生成量的影响;格里斯试剂法分析各组一氧化氮(NO)生成量;实时荧光定量聚合酶链反应(RT-PCR)观察各组PNPLA3和PPARαmRNA的相对含量,免疫印迹实验(Western blotting)检测各组PNPLA3、SREBP-1c、PPARα、Nrf2、CYP1A1和gp78的相对生成量。结果5~20 mg·L^(-1) ACAE可显著降低油酸诱导的HepG2细胞TG、NO、PNPLA3 mRNA及其蛋白、SREBP-1c、CYP1A1、gp78的相对生成量,上调ROS、PPARαmRNA及其蛋白、Nrf2的相对含量。结论ACAE可有效调控油酸诱导HepG2细胞的脂代谢,其机制可能是通过抑制PNPLA3等关键蛋白来减少脂质堆积,以及通过激活Nrf2等信号通路来促进脂质氧化。Objective To investigate the effects of Allium cepa var.aggregatum extract(ACAE)on lipid metabolism of oleic acid-induced HepG2 cells and its underlying mechanism.Methods MTT was used to select the appropriate experimental concentration of ACAE,enzyme-linked immunosorbent assay(ELISA)was used to detect the effects of different concentrations of ACAE on the production of TG and ROS in oleic acid-induced HepG2 cells,and Griese reagent was used to analyze the difference of NO production in each group.Real-time PCR was used to observe the relative contents of PNPLA3 and PPARαmRNA in each group.Western blot was used to observe the relative production of PNPLA3,SREBP-1C,PPARα,Nrf2,CYP1A1,and GP78 in each group.Results In the concentration of 5-20 mg·L^(-1),ACAE significantly decreased the relative production of TG,NO,PNPLA3 mRNA and its corresponding protein,SREBP-1C,CYP1A1,GP78,and up-regulated the relative contents of ROS,PPARαmRNA and its corresponding protein,and Nrf2 in oleic acid-induced HepG2 cells.Conclusion ACAE could effectively regulate the lipid metabolism of oleic acid-induced HepG2 cells,and the mechanism might be related to reducing lipid accumulation by inhibiting key proteins such as PNPLA3 and promoting lipid oxidation by activating signaling pathways such as Nrf2.

关 键 词：葱白提取物 油酸 脂代谢 氧化应激 

分 类 号：R285[医药卫生—中药学] R972.6[医药卫生—中医学]