人参多糖对肠上皮屏障的增强作用及其机制  

Enhancing Effect and Mechanism of Panax ginseng Polysaccharide on Intestinal Epithelial Barriers

作　　者：刘乙婷 朱惠彬 陈婉霞 王安荣 胡玲[1] 李茹柳[1] LIU Yiting;ZHU Huibin;CHEN Wanxia;WANG Anrong;HU Ling;LI Ruliu(Science and Technology Innovation Center,Pi-Wei Institute,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学科技创新中心、脾胃研究所,广州510405

出　　处：《医药导报》2023年第9期1332-1339,共8页Herald of Medicine

基　　金：国家自然科学基金资助项目(81673940);广州市科技计划项目(201607010335);广州中医药大学一流学科重点项目(广中医校办[2020]62号)。

摘　　要：目的观察人参多糖对大鼠小肠上皮细胞(IEC-6)渗透性、黏附连接蛋白表达及细胞钙离子(Ca^(2+))水平的影响,探讨益气健脾中药人参肠黏膜保护作用机制。方法设置不同实验条件[正常含钙培养、含钙培养+二氟甲基鸟氨酸(DFMO)、无钙培养]以观察人参多糖的作用;Transwell小室酚红透过法检测细胞间渗透性;Western blotting法检测黏附连接蛋白(E-cadherin、α-catenin和β-catenin)表达;激光共聚焦显微镜观察细胞Ca^(2+)水平。结果①正常含钙培养时,人参多糖(40、80、160 mg·L^(-1),下同)能降低细胞间渗透性、提高E-cadherin、α-catenin和β-catenin表达及细胞Ca^(2+)水平(P<0.05或P<0.01);②含钙培养+DFMO负荷时,细胞间渗透性增加、E-cadherin和α-catenin表达及Ca^(2+)水平降低(P<0.05或P<0.01),人参多糖可改善DFMO所致的细胞间渗透性增加、E-cadherin和α-catenin表达及Ca^(2+)水平降低(P<0.05或P<0.01);③无钙培养可致细胞间渗透性增加及E-cadherin、α-catenin和β-catenin表达降低(P<0.05或P<0.01),人参多糖可改善无钙培养所致的细胞间渗透性增加及E-cadherin和α-catenin表达下降(P<0.05或P<0.01)。结论人参多糖具有增强肠上皮屏障的作用,其机制与提高细胞Ca^(2+)水平及黏附连接蛋白表达有关。Objective To investigate the potential effect of Panax ginseng polysaccharide on the permeability,the expression of adhesion junction proteins,and calcium ion level in rat small intestinal epithelial cells(IEC-6),and to explore the mechanism of Chinese herbs with the actions of strengthening qi and invigorating spleen in intestinal mucosal protection.Methods Different experimental conditions[normal Ca^(2+)-containing culture,Ca^(2+)-containing+DFMO(difluoromethylornithine,polyamine synthesis inhibitors)culture,and Ca^(2+)-free culture]were set to investigate the effect of Panax ginseng polysaccharide;the transwell cell phenol red permeability was measured to observe the intercellular permeability;Western blotting was used to determine the expression of adhesion junction proteins(E-cadherin,α-catenin,andβ-catenin);laser confocal microscopy was used to observe the level of intracellular calcium.Results①When cultured in normal Ca^(2+)-containing medium,the result showed that Panax ginseng polysaccharide(40,80 or 160 mg·L^(^(-1)),below)can reduce intercellular permeability,improve the expression of adhesion junction proteins(E-cadherin,α-catenin andβ-catenin)and the level of intracellular calcium(P<0.05 or P<0.01);②In Ca^(2+)-containing+DFMO culture,the intercellular permeability increased and the expression of adhesion junction proteins(E-cadherin,α-catenin)and the level of intracellular calcium decreased(P<0.05 or P<0.01),Panax ginseng polysaccharide could ameliorate the increase of intercellular permeability and the reduction of adhesion junction proteins(E-cadherin,α-catenin)and the level of intracellular calcium induced by DFMO(P<0.05 or P<0.01);③In Ca^(2+)-free culture,the intercellular permeability increased and the expression of adhesion junction proteins(E-cadherin、α-catenin andβ-catenin)and the level of intracellular calcium decreased(P<0.05),Panax ginseng polysaccharide could ameliorate the increase of intercellular permeability and decrease of adhesion junction proteins(E-cadherin,α-cate

关 键 词：人参多糖 细胞间渗透性 钙离子 黏附连接蛋白 上皮屏障 

分 类 号：R285.5[医药卫生—中药学] R256.3[医药卫生—中医学]