Immuno-Protective Efficiency of the Bivalent Inactivated Vaccine Against Vibrio scophthalmi and Aeromonas salmonicida Infections in Turbot(Scophthalmus maximus L.)  

作　　者：ZHOU Shun ZHENG Xujia DING Yuanyuan SU Lin HUANG Qing XIU Yunji 

机构地区：[1]School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Journal of Ocean University of China》2023年第4期1079-1086,共8页中国海洋大学学报（英文版）

基　　金：supported by the Fish Innovation Team of Shandong Agriculture Research System (No. SDAIT-1206);the Aquatic Animal Immunologic Agents Engineering Research Center of Shandong Province, the Qingdao Agricultural University Doctoral Start-Up Fund (6631122030);the National Natural Science Foundation of China (No. 32002421);the Advanced Talents Foundation of QAU (No. 6651118016);the Natural Science Foundation of Shandong Province (No. ZR2019BC009);the ‘First-Class Fishery Discipline’ program of Shandong Province, the special top talent plan ‘One Thing One Decision (Yi Shi Yi Yi)’;the Key Research and Development Program in Shandong Province (No. 2018YFJH0703);Breeding Plan of Shandong Provincial Qingchuang Research Team (2019)

摘　　要：Vibrio scophthalmi and Aeromonas salmonicida can cause high turbot mortality and huge economic losses.Presently,vaccination is the most promising method for preventing communicable diseases.In this study,we used formalin to kill V.scophthalmi and A.salmonicida cells,and mixed with the mineralized oil adjuvant(Montanide^(TM)ISA 763 AVG)to prepare the bivalent inactivated vaccine.The results showed that turbot inoculated with the bivalent inactivated vaccine exhibited strong tolerance to the infection of V.scophthalmi and A.salmonicida,and no obvious clinical symptoms and pathological changes were observed.The activities of enzymes lysozyme,acid phosphatase and complement C3 had significantly increased after the vaccination.The antibody titer response of vaccinated turbot was greatly boosted,which was positively connected with the immunological impact according to ELISA results.Simultaneously,the expression levels of immune-related genes such as MHC-IIα,MHC-IIβ,CD4,CD8,TNF-αand IL^(-1)βwere up-regulated,demonstrating that it might stimulate humoral and cellular immunological response in turbot.These findings highlight the potential of the bivalent inactivated vaccine for controlling V.scophthalmi and A.salmonicida infections in turbot.

关 键 词：Aeromonas salmonicida Vibrio scophthalmi bivalent inactivated vaccine immune response 

分 类 号：S943[农业科学—水产养殖]