Transcriptome Analysis of Mantle Tissues Reveals Potential Shell-Matrix-Protein Genes in Gigantidas haimaensis  

作　　者：SHI Yu YAO Gaoyou HE Maoxian 

机构地区：[1]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou,Guangzhou 511458,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Ocean University of China》2023年第4期1087-1097,共11页中国海洋大学学报（英文版）

基　　金：supported by the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory,Guangzhou,China(No.GML2019ZD0401);the Major Project of Basic and Applied Basic Research of Guangdong Province(No.2019 B030302004);the Science and Technology Planning Project of Guangdong Province,China(No.2020B1212060058).

摘　　要：Haima cold seep ecosystem is on the northwestern slope of the South China Sea,which is characterised by high pressure,low temperature,hypoxia,and low pH value.The deep-sea mussel Gigantidas haimaensis is distributed in this ecosystem.Previous studies have focused on its adaptation to abiotic stress,biogeography,ecotoxicology,genomes,immunity and symbiosis,but knowledge on biomineralisation remains lacking.Herein,we generated a comprehensive transcript dataset from G.haimaensis mantle tissue,and 30255 unigenes were assembled.The top 20 most highly expressed genes are related to energy supply,such as mitochondrial genes,suggesting they may mediate the adaptability of this deep-sea mussel to the high pressure and hypoxia environment.Eleven shell matrix protein(SMP)-related genes were identified from the transcriptome data.Quantitative PCR analyses showed that five of ten acidic SMPs and nacreous-layer-matrix-protein genes(nacrein,perlucin,perlwapin,pif and mantle protein)were highly expressed in mantle tissue,while the expressions of other five genes(chitinase,SPARC,TRY,papilin and calmodulin)were low.Scanning electron microscopy showed that the shell was composed of a prismatic layer and a nacreous layer,and every nacreous layer was made of the whole pieces of aragonite that stacked on top of each other.These results indicated the conservation of the structure and functions of nacreous matrix genes in G.haimaensis.Moreover,the nacreous layer was made of whole pieces of aragonite that were not quadrilateral or polygonal pieces.Studying these genes will likely reveal the molecular mechanisms of biomineralisation in G.haimaensis and other deep-sea mussels.

关 键 词：Gigantidas haimaensis TRANSCRIPTOME BIOMINERALISATION MANTLE shell structure 

分 类 号：Q953[生物学—动物学]