2.9%吡唑醚菌酯·精甲霜灵·甲维盐种子处理悬浮剂高效液相色谱分析  

HPLC analysis of pyraclostrobin·metalaxyl-M·emamectin benzoate 2.9%FS

作　　者：吴曼 王胜得 李涛 杜升华 陈明 WU Man;WANG Shengde;LI Tao;DU Shenghua;CHEN Ming(National Engineering Research Center for Agrochemicals,Hunan Research Institute of Chemical Industry,Changsha 410014,China;Hunan Haili Chemical Industry Co.,Ltd.,Changsha 410007,China;Beinong(Haili)Zhuozhou Seed-coating Co.,Ltd.,Baoding 072750,Hebei,China)

机构地区：[1]湖南化工研究院有限公司国家农药创制工程技术研究中心,长沙410014 [2]湖南海利化工股份有限公司,长沙410007 [3]北农(海利)涿州种衣剂有限公司,河北保定072750

出　　处：《农药》2023年第8期576-578,598,共4页Agrochemicals

摘　　要：[目的]建立种子处理悬浮剂中吡唑醚菌酯、精甲霜灵和甲维盐3种有效成分同时测定的高效液相色谱分析方法。[方法]选用C_(18)色谱柱,选择225 nm为检测波长,在甲醇-0.3%氨水的流动相条件下,采用梯度洗脱方式对试样中吡唑醚菌酯、精甲霜灵和甲维盐进行有效分离和定量检测。[结果]方法中吡唑醚菌酯、精甲霜灵和甲维盐的线性相关系数分别为0.99999、0.99984、0.99970,标准偏差为0.015、0.016、0.019,相对标准偏差为0.82%、3.15%、3.09%,平均添加回收率分别为99.14%、99.65%、100.18%。[结论]方法线性良好,精密度高,满足该复配制剂的快速准确质量控制检测。[Aims]A method was established for simultaneous analysis of pyraclostrobin,metalaxyl-M,emamectin benzoate in suspension concentrate for seed treatment by HPLC.[Methods]The effective separation and quantification of active ingredients in the samples were performed on a C_(18)column by HPLC gradient elution method.The mobile phase was methanol and 0.3%ammonia aquous solution,and the detection wavelength was 225 nm.[Results]Within the detection methods of pyraclostrobin,metalaxyl-M and emamectin benzoate,the linear correlation coefficients were 0.99999,0.99984 and 0.99970,the standard deviations were 0.015,0.016 and 0.019,RSDs were 0.82%,3.15%and 3.09%,and the average recovery rate were 99.14%,99.65%and 100.18%,respectively.[Conclusions]The method with good linearity and high precision,meets the requirements of rapid and accurate quality control detection of the composition preparation.

关 键 词：吡唑醚菌酯 精甲霜灵 甲维盐 高效液相色谱 

分 类 号：TQ450.7[化学工程—农药化工]