氰霜唑及其主要代谢物CCIM在小白菜上的残留行为及膳食风险评估  

Residue behavior and dietary assessment of cyazofamid and its main metabolite CCIM in pakchoi

作　　者：陈秀[1] 张颂函 黄兰淇[1] 张正炜[1] 高永东[1] 温广月[2] 董茂峰 赵莉[1] CHEN Xiu;ZHANG Songhan;HUANG Lanqi;ZHANG Zhengwei;GAO Yongdong;WEN Guangyue;DONG Maofeng;ZHAO Li(Agriculture Technology Extension Service Center of Shanghai,Shanghai 201103,China;Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)

机构地区：[1]上海市农业技术推广服务中心,上海201103 [2]上海市农业科学院,上海201106

出　　处：《农药》2023年第8期588-593,共6页Agrochemicals

基　　金：上海市特色小宗作物安全绿色用药技术研究与示范(项目编号:沪农科推字(2022)第2-4号)。

摘　　要：[目的]为了评价氰霜唑在小白菜上的使用安全性,在上海、贵州、山东等10地进行了田间残留试验,研究氰霜唑及其主要代谢物4-氯-5-(4-甲苯基)-1H-咪唑-2-腈(CCIM)在小白菜中的残留行为,并进行膳食风险评估。[方法]小白菜样品中的氰霜唑和CCIM采用乙腈涡旋提取,提取液经盐析离心后,过滤膜,经液质联用仪检测,保留时间和碎片离子对定性,外标法定量。[结果]在0.005~1 mg/L的质量浓度范围内,在空白小白菜基质中氰霜唑和CCIM质量浓度与其仪器响应均呈良好线性关系,决定系数为0.996~0.999。通过添加回收率试验,在0.01、0.1、10 mg/kg 3个添加水平下,小白菜中氰霜唑及其代谢物的方法平均回收率和精密度分别为74%~85%和3%~7%。氰霜唑及CCIM的定量限均为0.01 mg/kg。20%氰霜唑悬浮剂在小白菜上以施药剂量300 g a.i./hm^(2)按推荐使用方法施药3次,末次药后7 d,氰霜唑、CCIM和氰霜唑(总)在小白菜中残留量分别为0.370~3.42、0.192~1.67、0.662~5.91 mg/kg,氰霜唑在小白菜中的消解动态符合一级动力学方程,降解半衰期为2.5~3.7 d。膳食风险评估结果表明,不同采收间隔期通过小白菜摄入氰霜唑对我国不同年龄段人群的急性和慢性膳食暴露风险均小于100%,风险可接受。推荐采收间隔期为7 d。[结论]为氰霜唑在小白菜上的安全性评估和科学合理使用提供指导。[Aims]In order to evaluate the application safety of cyazofamid in pakchoi,standardize residual filed trials in 10 places such as Shanghai,Guizhou,and Shandong were conducted to investigate the residue behavior of cyazofamid and its main metabolite 4-chloro-5-p-tolylimidazole-2-carbonitrile(CCIM).Dietary risk was also evaluated in this paper.[Methods]The samples were extracted by acetonitrile with vortex,the extracted solution was salting out and centrifuged,filtered through membrane,and then detected by LC-MS/MS.The retention time and fragment ion pairs were used for qualitative analysis,and the external standard method was used for quantitative analysis.[Results]Good linear relationships between the response and the concentration of cyazofamid and CCIM were observed in the range of 0.005-1 mg/L with the correlation coefficient of 0.996-0.999.The recoveries of cyazofamid and CCIM in pakchoi ranged from 74%to 85%with the RSDs of 3%-7%at the spiked levels of 0.01,0.1 and 10 mg/kg.The limits of quantification(LOQs)were both 0.01 mg/kg for cyazofamid and CCIM.The dissipation dynamics of cyazofamid(total)in pakchoi fitted the first-order kinetics equation with half-lives ranged from 2.5 to 3.7 d.Cyazofamid 20%SC was applied 3 times at the dosage of 300 g a.i./ha on pakchoi.The terminal residues of cyazofamid,CCIM and cyazofamid(total)in pakchoi were 0.370-3.42,0.192-1.67 and 0.662-5.91 mg/kg respectively with the pre-harvest interval of 7 d.The risk assessment results showed that acute and chronic dietary exposure risk were less than 100%for people at different ages,which were acceptable.The pre-harvest interval of 7 d was recommended.[Conclusions]This study provides guidance for the safety evaluation and rational application of cyazofamid in pakchoi.

关 键 词：小白菜 氰霜唑 残留 膳食风险评估 

分 类 号：TQ450.2[化学工程—农药化工]