九味楮实方对雌性CPP大鼠不良心理行为及GnRH表达的影响  

Effects of Jiuweichushi Formula on Adverse Psychological Behavior and GnRH Expression in Female CPP Rats

作　　者：黄田田 刘建忠[2] 冯小剑 HUANG Tiantian;LIU Jianzhong;FENG Xiaojian(First Clinical College,Hubei University of Chinese Medicine,Wuhan Hubei 430061,China;不详)

机构地区：[1]湖北中医药大学第一临床学院,湖北武汉430061 [2]湖北省中医院儿科 [3]中部战区总医院康复科

出　　处：《联勤军事医学》2023年第6期460-464,482,共6页Military Medicine of Joint Logistics

基　　金：湖北省自然科学基金一般面上项目(2020CFB825)。

摘　　要：目的探讨九味楮实方对中枢性性早熟(central precocious puberty,CPP)大鼠不良心理行为及促性腺激素释放激素(gonadotrophin releasing hormone,GnRH)的影响。方法将48只26日龄SD大鼠随机分为正常组,模型组,西药组,九味楮实方高、中、低剂量组,每组各8只。采用N-甲基-DL-天冬氨酸(N-methyl-dl-aspartic acid,NMA)皮下注射建立CPP大鼠模型;造模同时,西药组皮下注射戈那瑞林干预,九味楮实方高、中、低剂量组分别给予不同剂量的九味楮实方灌胃干预。观察大鼠的阴道口开放所需时间,并进行强迫游泳实验(forced swim test,FST)、糖水偏好实验(sucrose preference test,SPT)。采用实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,qPCR)和Western blot实验检测各组大鼠下丘脑GnRH基因及蛋白表达水平。结果相较于正常组,模型组大鼠阴道口开启时间提前,组间比较差异有统计学意义(P<0.01);与模型组比较,西药组及九味楮实方高、中剂量组大鼠阴道口开启时间均延迟,组间比较差异有统计学意义(P均<0.05)。与正常组比较,模型组大鼠强迫游泳不动时间增加,糖水消耗率降低,组间比较差异有统计学意义(P均<0.05);与模型组比较,西药组及九味楮实方高、中、低剂量组大鼠强迫游泳不动时间均缩短,组间比较差异有统计学意义(P均<0.05)。与正常组比较,模型组大鼠下丘脑GnRH基因及蛋白表达水平均升高,组间比较差异有统计学意义(P均<0.01);与模型组比较,西药组及九味楮实方高、中剂量组大鼠下丘脑GnRH基因及蛋白表达水平均降低,组间比较差异有统计学意义(P均<0.01)。结论九味楮实方可以延缓CPP大鼠阴道口开放,抑制下丘脑GnRH分泌,并改善CPP大鼠不良心理行为。Objective To investigate the effects of Jiuweichushi formula on adverse psychological behavior and gonadotrophin releasing hormone(GnRH)in rats with central precocious puberty(CPP).Methods A total of 4826-day-old SD rats were randomly divided into normal group,model group,western medicine group,Jiuweichushi formula high-dose,medium-dose and low-dose groups,with 8 rats in each.The CPP model was established by subcutaneous injection of N-methyl-dl-aspartic acid(NMA);while building the model,subcutaneous injection of gonarellin was performed in the western medicine group,the Jiuweichushi formula high-dose,medium-dose and low-dose groups were given different doses of Jiuweichushi formula by gavage.The opening time of the vaginal orifice of the rats was observed,forced swim test(FST)and sucrose preference test(SPT)were carried out.The expression levels of GnRH gene and protein expression in hypothalamus were detected by real-time fluorescent quantitative polymerase chain reaction(qPCR)and Western blot.Results Compared with the normal group,the opening time of the vaginal orifice of the rats in the model group was significantly earlier,and the difference between groups was statistically significant(P<0.01);compared with the model group,the opening time of the vaginal orifice of the rats in the western medicine group and the Jiuweichushi formula highdose as well as medium-dose groups were significantly delayed,and the difference among groups was statistically significant(all P<0.05).Compared with the normal group,the forced swimming immobility time of the model group significantly increased,the sugar consumption rate significantly decreased,and the difference between groups was statistically significant(all P<0.05);compared with the model group,the forced swimming immobility time of the western medicine group and the Jiuweichushi formula high-dose,medium-dose as well as low-dose groups were significantly shortened,and the difference among groups was statistically significant(all P<0.05).Compared with the normal group,th

关 键 词：中枢性性早熟 不良心理行为 九味楮实方 促性腺激素释放激素 

分 类 号：R272[医药卫生—中医儿科学]