姜黄素调控JAK2/STAT3通路参与重症急性胰腺炎动物模型肾脏细胞凋亡的研究  

Regulation of JAK2/STAT3Pathway by Curcumin in Renal Cell Apoptosis in Animal Model with Severe Acute Pancreatitis

作　　者：阿尔帕提·买买提[1] 地力木热提·艾买提 郭飞[1] AERPATI Maimaiti;DILIMURETI Aimaiti;GUO Fei(Department of Emergency Trauma,the First Affiliate Hospital of Xinjiang Medical University,Urumchi Xinjiang 830001,China;不详)

机构地区：[1]新疆医科大学第一附属医院急诊创伤外科,新疆乌鲁木齐830001 [2]新疆医科大学附属中医医院普外一科

出　　处：《联勤军事医学》2023年第6期465-471,共7页Military Medicine of Joint Logistics

基　　金：新疆维吾尔自治区自然科学基金(2019D01C304)。

摘　　要：目的探究姜黄素调控Janus激酶2(Janus kinase 2,JAK2)/信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)通路参与重症急性胰腺炎(severe acute pancreatitis,SAP)肾脏细胞凋亡。方法将58只SPF级别大鼠适应性饲养后,分成假手术组(n=10)、模型组(n=10)、姜黄素低剂量组(n=9)、姜黄素高剂量组(n=10)、阳性对照组(n=10)及JAK2/STAT3抑制组(n=9)。各组相应药物干预后,测量不同组别大鼠胰腺组织湿质量比和腹水量。苏木素-伊红(hematoxylin-eosin,HE)染色法测定不同组别大鼠肾脏组织病理变化;全自动生化分析仪测定血清淀粉酶(amylase,AMY)、肌酐(creatinine,Cr)和血尿素氮(blood urea nitrogen,BUN);酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)测定白细胞介素6(interleukin-6,IL-6)、白细胞介素1β(interleukin-1 beta,IL-1β)和肿瘤坏死因子α(tumor necrosis factor alpha䥺Symboll@@TNF-α)水平;末端脱氧核苷酸转移酶介导的dUTP切口及末端标记(terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling,TUNEL)技术测定肾脏细胞凋亡情况;反转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)测定肾脏B淋巴细胞白血病-2(B-cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2-associated X protein,Bax)、半胱氨酸天门冬酰胺蛋白酶3(cysteine-aspartic protease 3,Caspase-3)的mRNA表达;Western blot法测定肾脏细胞磷酸化磷酸化酪氨酸激酶2(phosphorylated Janus kinase 2,p-JAK2)、磷酸化信号转导与转录激活因子3(phosphorylated signal transducer and activator of transcription 3,p-STAT3)蛋白表达。结果不同组大鼠胰腺组织湿质量比和腹水量比较差异具有统计学意义(P均<0.001)。假手术组大鼠胰腺组织结构清晰、形态正常,且无水肿、炎症细胞浸润;模型组大鼠胰腺组织水肿、空泡化样变,存在炎症细胞浸润或腺泡细胞坏死;姜黄素不同剂量组、阳性对照组及JAK2/Objective To investigate the regulation of Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)pathway by curcumin in renal cell apoptosis in animal model with severe acute pancreatitis(SAP).Methods After adaptive feeding 58 SPF grade rats,they were divided into sham surgery group(n=10),model group(n=10),curcumin low-dose group(n=9),curcumin high-dose group(n=10),positive control group(n=10)and JAK2/STAT3 inhibition group(n=9).After drug intervention,wet mass ratio and abdominal water volume of pancreatic tissue were measured in each group.The pathological changes of renal tissues of rats in different groups was determined by hematoxylin-eosin(HE)staining;determination of serum amylase(AMY),creatinine(Cr)and blood urea nitrogen(BUN)were measured by fully automatic biochemical analyzer;the levels of interleukin-6(IL-6),interleukin-beta(IL-1β)and tumor necrosis factor alpha(TNF-α)were measured by enzyme-linked immunosorbent assay(ELISA);the apoptosis of renal cells was determined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL);the mRNA expressions of renal B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax)and cystaine-aspartic protease 3(Caspase-3)were measured by reverse transcription-polymerase chain reaction(RT-PCR);the expressions of phosphorylated Janus kinase 2(p-JAK2)in renal cells and phosphorylated signal transducer and activator of transcription 3(p-STAT3)protein were measured by Western blot.Results There were significant differences in wet mass ratio and abdominal water volume of pancreatic tissue of rats among different groups(all P<0.001).In the sham surgery group,the pancreatic tissue was clear and normal,and there was no edema and inflammatory cell infiltration;in model group,pancreatic tissue edema,vacuolation,inflammatory cell infiltration or acinar cell necrosis existed;the histopathological changes of pancreas could be improved in different curcumin dose group,positive control group and JAK2/STAT3 inhibition group.There wer

关 键 词：姜黄素 重症急性胰腺炎 细胞凋亡 Janus激酶2/信号转导和转录激活因子3 

分 类 号：R657.51[医药卫生—外科学]