健康体检儿童肺炎支原体检出情况研究  

Prevalence of Mycoplasma pneumoniae among children undergoing physical examination

作　　者：李少丽[1] 刘立雍 黄乐乐 刘洁[2] 王磊 党荔[4] 向才鑫 杨颖 赵飞[2] Li Shaoli;Liu Liyong;Huang Lele;Liu Jie;Wang Lei;Dang Li;Xiang Caixin;Yang Ying;Zhao Fei(Translational Medicine Department,Capital Institute of Pediatrics,Beijing 100020,China;State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of Respiratory Medicine,Children′s Hospital,Capital Institute of Pediatrics,Beijing 100020,China;Outpatient Treatment Center,Children′s Hospital,Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所转化医学研究室,北京100020 [2]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206 [3]首都儿科研究所附属儿童医院呼吸科,北京100020 [4]首都儿科研究所附属儿童医院门诊治疗中心,北京100020

出　　处：《中华微生物学和免疫学杂志》2023年第7期555-558,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(32070188)。

摘　　要：目的了解参与健康体检儿童人群的肺炎支原体(Mycoplasma pneumoniae,Mp)检出情况。方法随机入组2023年参与健康体检的6~12岁小学生1303人,采集受试者口咽拭子及静脉血标本,使用分离培养、核酸检测以及血清学抗体方法检测受试者Mp检出情况,并进行统计学分析。结果1303例健康儿童中Mp培养阳性检出率为4.1%(53/1303),核酸阳性检出率为7.3%(95/1303),血清学IgM抗体阳性检出率为13.6%(177/1303)。经统计学分析,健康儿童人群中Mp培养与核酸检测以及血清IgM抗体检测阳性率之间的差别均具有统计学意义(P<0.05)。结论2023年健康体检儿童中Mp检出率约为4.1%。分离培养方法对健康人群中Mp检出率检测准确性优于核酸以及血清学方法,核酸和血清学方法会高估正常人群中Mp检出率。Objective To investigate the prevalence of Mycoplasma pneumoniae(Mp)in children undergoing physical examination.Methods This study randomly enrolled 1303 children at the age of 6-12 years who underwent physical examination in 2023.Their oral and pharyngeal swabs as well as venous blood samples were collected.The prevalence of Mp in these subjects was detected using isolation and culturing,nucleic acid detection and serological test.Chi-square test was used for statistical analysis.Results Among the 1303 children,the detection rate of Mp was 4.1%(53/1303)by culturing,7.3%(95/1303)by nucleic acid detection and 13.6%(177/1303)by serological test.Statistical analysis showed that there were significant differences in the the detection rates of Mp among children undergoing physical examination between the three methods(P<0.05).Conclusions The detection rate of Mp in children undergoing physical examination in 2023 was about 4.1%.Isolation and culturing was more accurate than nucleic acid detection and serological test in the detection of Mp in healthy population as the latter two methods would overestimate the rate.

关 键 词：肺炎支原体 健康人群 检出率 分离培养 

分 类 号：R725.6[医药卫生—儿科] R446.5[医药卫生—临床医学]