藤梨根乙醇提取物诱导小细胞肺癌凋亡的机制研究  

Mechanism of Tengligen ethanol extract inducing apoptosis in small cell lung cancer

作　　者：张田革 赵周锴 Nishimwe Ange 王晓戎[3] 吴志浩[1,2] ZHANG Tiange;ZHAO Zhoukai;NISHIMWE Ange;WANG Xiaorong;WU Zhihao(Research Laboratory of Tumor Microenvironment,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院肿瘤微环境研究室,安徽芜湖241002 [2]皖南医学院医学生物学教研室,安徽芜湖241002 [3]安徽中医药高等专科学校中医学系,安徽芜湖241002

出　　处：《皖南医学院学报》2023年第4期320-323,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81872371);安徽省自然科学基金项目(1708085MH203);分子肿瘤学国家重点实验室开放课题(SKL-KF-2019-11);安徽省高校学科(专业)拔尖人才学术资助(gxbjZD2020110)。

摘　　要：目的:研究藤梨根乙醇提取物(TLG)对H1688、H446细胞凋亡的影响,并探究其相关的分子机制。方法:用不同浓度TLG处理H446、H1688细胞:使用MTT法检测细胞活力;流式细胞仪检测细胞凋亡状况;Western blot检测Erk1/2、Foxo3a、PUMA、Cleaved PAr P蛋白表达量。结果:经TLG处理后,与不加药组相比,细胞的增殖抑制率以剂量依赖性形式增加;细胞凋亡率增加;Western Blot显示Erk1/2蛋白表达量随着TLG浓度的增加而降低;Foxo3a、PUMA、Cleaved PAr P蛋白表达量随着TLG浓度的增加而增加。结论:TLG可促进小细胞肺癌细胞H1688、H446的凋亡,其机制可能是通过Erk1/2-Foxo3a-PUMA轴发挥作用。Objective:To investigate the effect of ethanol extract of Tengligen(TLG)on apoptosis of small cell lung cancer (SCLC)cells H1688 and H446 and the potentia molecular mechanism.Methods:H446 and H1688 cells were treated with different concentration of ethanol extract of TLG.Cell viability was measured with MTT assay.Cell apoptosis was determined by flow cytometry.Western Blot was used to detect the expression of extracellular regulated protein kinase(Er K1/2),forkhead box O3(Foxo3a),p53 up-regulated modulator of apoptosis(PUMA)and cleaved poly ADP-ribose polymerase(PAr P).r esults:Compared with untreated group,cell proliferation inhibition rate was increased in a dose-dependent manner.Cell apoptosis rate was also increased.Western blot showed that protein expression of Erk1/2 down-regulated was decreased with added ethanol extract concentration.Protein Foxo3a,PUMA,cleaved PAr P expression was increased with the increase of ethanol extract concentration.Conclusion:TLG ethanol extract can promote the apoptosis of SCLC cells H1688 and H446 via possible mechanism of Erk1/2-Foxo3a-PUMA axis.

关 键 词：藤梨根乙醇提取物 细胞凋亡 ERK1/2 小细胞肺癌 

分 类 号：R285[医药卫生—中药学]