邻苯二甲酸二乙基己酯体内暴露对小鼠受精卵发育潜能的影响  

Effects of in vivo exposure to di-2-ethylhexyl phthalate on developmental potential of mouse zygotes

作　　者：赵华[1] 王婷[2] 王璐[1] 万锋 冯科[1] 韦多[1] 张翠莲[1] ZHAO Hua;WANG Ting;WANG Lu;WAN Feng;FENG Ke;WEI Duo;ZHANG Cuilian(Reproductive Medicine Center,Henan Provincial People′s Hospital,Zhengzhou 450003;Reproductive Medicine Center,Henan University People′s Hospital,Zhengzhou 450003)

机构地区：[1]河南省人民医院生殖医学中心,郑州450003 [2]河南大学人民医院生殖中心,郑州450003

出　　处：《郑州大学学报（医学版）》2023年第4期454-458,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81701444);河南卫健委科技创新杰出青年人才培养项目(YXKC2020021);河南省医学科技攻关省部共建重点项目(SBGJ202102016)。

摘　　要：目的:探讨邻苯二甲酸二乙基己酯(DEHP)体内暴露对小鼠胚胎卵裂、囊胚形成、胚胎着床及胚胎线粒体膜电位的影响。方法:将40只3周龄清洁级ICR雌性小鼠(雌鼠)随机分为DEHP低剂量(300 mg/kg)组、DEHP中剂量(600 mg/kg)组、DEHP高剂量(1200 mg/kg)组和对照组,每组10只。采用灌胃方式构建DEHP暴露小鼠模型,按照5 mL/kg给药,每周5 d,每天1次,连续染毒8周。对照组灌胃玉米油。将各组雌鼠促排卵与非染毒雄性小鼠合笼后,取受精卵行胚胎培养,比较各组胚胎卵裂率、囊胚形成率、胚胎着床位点数和胚胎线粒体膜电位的差异。结果:DEHP低、中、高剂量组小鼠胚胎卵裂率、囊胚形成率及胚胎着床位点数均低于对照组(P<0.05),随着DEHP暴露浓度的升高,囊胚形成率下降(P<0.05);DEHP低、中、高剂量组小鼠胚胎线粒体膜电位均低于对照组(P<0.001)。结论:DEHP体内暴露对早期胚胎发育有毒性作用。Aim:To investigate the effects of in vivo exposure to di-2-ethylhexyl phthalate(DEHP)on embryo cleavage,blastocyst formation,embryo implantation and mitochondrial membrane potential.Methods:Forty 3-week-old clean grade ICR female mice were allocated into DEHP low dose(300 mg/kg)group,DEHP medium dose(600 mg/kg)group,DEHP high dose(1200 mg/kg)group and the control group,with 10 mice in each group.The DEHP exposed mouse model was orally administered with DEHP once per day for 8 weeks(5 mL/kg,5 days per week).The control group was given corn oil.The ovulation induced female mice were caged with non-exposed male mice,and the zygotes were collected to compare the differences of embryo cleavage rate,blastocyst formation rate,implantation sites and embryonic mitochondrial membrane potential among the groups.Results:The embryo cleavage rate,blastocyst formation rate and embryo implantation sites of the DEHP low,medium and high dose groups were significantly lower than those of control group(P<0.05),and the blastocyst formation rate was significantly decreased with the increase of exposure dose(P<0.05);the mitochondrial membrane potential of embryo in the DEHP low,medium and high dose groups was significantly lower than that in the control group(P<0.001).Conclusion:Exposure to DEHP in vivo may has toxic effects on early embryonic development.

关 键 词：邻苯二甲酸二乙基己酯 体内暴露 线粒体膜电位 胚胎发育 小鼠 

分 类 号：R711.6[医药卫生—妇产科学]