5个绵羊品种DLK1基因多态性与生长性状的关联性分析  

Correlation Analysis Between DLK1 Gene Polymorphism and Growth Traits in Five Sheep Breeds

作　　者：孟泉禄 张明军[1] 史金平 付玲娟 刘婷[1] 张全伟[2] 成述儒[1] MENG Quanlu;ZHANG Mingjun;SHI Jinping;FU Lingjuan;LIU Ting;ZHANG Quanwei;CHENG Shuru(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《华北农学报》2023年第4期233-238,共6页Acta Agriculturae Boreali-Sinica

基　　金：甘肃农业大学学科建设基金(GAU-XKJS-2018-024);农业行业专项(201303062-5);甘肃农业大学学科团队项目(GAU-XKTD-2022-21)。

摘　　要：为挖掘与绵羊生长性状相关的分子标记基因,探究DLK1多态性与绵羊生长性能之间的关联性。以240头不同品种绵羊(甘肃高山细毛羊、蒙古羊、藏绵羊和小尾寒羊各50头,滩羊40头)为试验对象,分别测定不同品种各年龄段绵羊生长性能指标;采集绵羊血液,提取DNA,运用PCR-SSCP方法结合DNA测序技术分析不同品种绵羊DLK1基因多态性;SPSS 20.0软件分析不同基因型与各绵羊品种不同年龄段生长性能之间的相关性。结果表明,不同品种绵羊DLK1基因均具有多态性,其中共检测到2个等位基因(A和B)和3种基因型(AA、AB和BB),优势等位基因和基因型分别为B和AB;DLK1基因第5内含子30412 bp处发生G→A单个碱基突变;G30412A突变位点与绵羊1月龄和3月龄的体质量和胸围具有显著相关性(P<0.05),且BB基因型个体表型优于AA基因型个体,突变位点与绵羊体长和体高无相关性(P>0.05)。总之,不同品种绵羊DLK1基因均存在第5内含子上G30412A突变位点,该位点显著影响绵羊1,3月龄的体质量和胸围,其可作为选择甘肃地方绵羊品种生产性状的候选基因。To explore the association between DLK1 polymorphism and growth performance in sheep.A total of 240 sheep of different breeds(Gansu alpine fine wool sheep,Mongolian sheep,Tibetan sheep,Small tailed han sheep,50 sheep each and Tan sheep,40 sheep)were selected as the experimental subjects.The growth performance indexes of different breeds of sheep at different ages were measured.Blood samples were collected and DNA was extracted.DLK1 gene polymorphisms were analyzed by PCR-SSCP combined with DNA sequencing.SPSS 20.0 software was used to analyze the correlation between different genotypes and growth performance of each sheep breed at different ages.The results showed that DLK1 gene was polymorphic in different breeds of sheep.Two alleles(A and B)and three genotypes(AA,AB and BB)were detected,and the dominant allele and genotype were B and AB,respectively.A single nucleotide mutation(G→A)was detected at the 30412 bp site in intron 5 of DLK1 gene.The G30412A mutation was associated with the weight and chest circumference of sheep at 1 and 3 months old.The phenotype of BB genotype sheep was better than that of AA genotype sheep.There was no correlation between the mutation sites and the body length and body height.The results of this study showed that the G30412A mutation in intron 5 of DLK1 gene existed in different breeds of sheep,and this mutation significantly affected the body weight and chest circumference of sheep at the 1 and 3 months old,which could be used as a candidate gene for the production traits of Gansu sheep breeds.

关 键 词：绵羊 生长性状 DLK1基因 多态性 PCR-SSCP 

分 类 号：S826[农业科学—畜牧学]