柠檬酸和醋酸对小鼠离体肠平滑肌运动的影响  

Effects of Citric Acid and Acetic Acid on the Contraction of Isolated Intestinal Smooth Muscle in Mice

作　　者：周鑫漪 范桢 岳家田 刘馨博 赵红琼[1] ZHOU Xinyi;FAN Zhen;YUE Jiatian;LIU Xinbo;ZHAO Hongqiong(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052

出　　处：《畜牧与饲料科学》2023年第4期22-28,共7页Animal Husbandry and Feed Science

基　　金：新疆维吾尔自治区大学生创新训练计划项目(S202210758063)。

摘　　要：[目的]探究柠檬酸和醋酸对小鼠离体肠平滑肌运动的影响及可能的机制,为开发具有调控人或动物消化道运动机能障碍的有机酸类产品提供参考。[方法]用生物信号处理系统监测2倍浓度递增柠檬酸(0.02~0.64 mmol/L)或醋酸(0.13~2.00 mmol/L)及其一次性有效浓度对小鼠离体肠段收缩幅度、收缩张力及峰值的影响。[结果]柠檬酸和醋酸均剂量依赖性地减小肠平滑肌的收缩幅度和收缩张力(P<0.01),其有效抑制浓度分别为0.32 mmol/L和1.00 mmol/L。有效抑制浓度的柠檬酸和醋酸均不影响25 mmol/L KCl引起的小肠收缩峰值(P>0.05),但柠檬酸处理后显著(P<0.05)缓解M-受体激动剂氯贝胆碱引起的肠收缩幅度减小。[结论]柠檬酸和醋酸对小鼠离体肠平滑肌运动均具有抑制作用,且可能并不影响平滑肌细胞膜的去极化过程,但柠檬酸可能通过部分影响M-受体的作用而对消化道平滑肌具有解痉作用。[Objective]This study was conducted to explore the effects and possible mechanisms of citric acid and acetic acid on the contraction of isolated mouse intestinal smooth muscle,and to provide references for the development of organic acid products that helps regulate gastrointestinal motility disorders in humans or animals.[Method]A biological signal processing system was used to monitor the effects of two-fold increasing concentrations of citric acid(0.02-0.64 mmol/L)or acetic acid(0.13-2.00 mmol/L)as well as their corresponding bolus effective concentrations on the contractile amplitude,ension,and peak of the isolated intestinal segment in mice.[Result]Both citric acid and acetic acid dose-dependently reduced the contractile amplitude and tension of the intestinal smooth muscle(P<0.01),with the effective inhibitory concentrations of 0.32 mmol/L and 1.00 mmol/L,respectively.Both acids with effective inhibitory concentrations did not affect the contractile peak of the intestinal tissue caused by 25 mmol/L KCl(P>0.05),while the citric acid treatment significantly(P<0.05)alleviated the decrease in intestinal contractile amplitude induced by bethanechol chloride,an M-receptor agonist.[Conclusion]Both citric acid and acetic acid can inhibit the contraction of isolated intestinal smooth muscle in mice,and may not affect the depolarization process of smooth muscle cell membrane.However,citric acid may have spasmolytic effect on gastrointestinal smooth muscle via partially affecting the role of M-receptor.

关 键 词：柠檬酸 醋酸 小鼠 肠 平滑肌 M-受体 

分 类 号：S858.91[农业科学—临床兽医学] Q414[农业科学—兽医学]