外源油菜素内酯引发对老化燕麦种子萌发特性的影响  

Effects of Exogenous Brassinosteroids Priming on Germination Characteristics of Aged Oat Seeds

作　　者：李尹琳 王勃 夏方山 张杰敏 张金成 李嘉俊 LI Yinlin;WANG Bo;XIA Fangshan;ZHANG Jiemin;ZHANG Jincheng;LI Jiajun(College of Grassland Science,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学草业学院,山西太谷030801

出　　处：《畜牧与饲料科学》2023年第4期94-100,共7页Animal Husbandry and Feed Science

基　　金：山西农业大学“十四五”生物育种工程项目(YZGC134);山西省重点研发计划项目(202102140601006);内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)。

摘　　要：[目的]探讨外源油菜素内酯(brassinosteroids,BR)引发对老化燕麦(Avena sativa)种子活力的影响。[方法]以人工老化后的燕麦种子为试验材料,经不同浓度(0、0.01、0.10、0.50、1.00、10.00μmol/L)的BR溶液引发0(CK)、1、6、12、18、24 h,分析其发芽率、发芽指数、平均发芽时间和幼苗活力指数的变化规律。[结果]不同外源BR浓度、引发时间以及两者之间的交互作用对老化燕麦种子发芽率、发芽指数、平均发芽时间、幼苗活力指数均存在极显著(P<0.01)影响。BR浓度为0.01μmol/L引发24 h时,老化燕麦种子的发芽率、发芽指数和幼苗活力指数均最高,平均发芽时间最短;在BR浓度为10.00μmol/L时,1~24 h老化燕麦种子的发芽率、发芽指数、幼苗活力指数均低于其他浓度,而平均发芽时间显著(P<0.05)高于其他浓度。[结论]低浓度外源BR引发能够提高老化燕麦种子活力水平,而高浓度则降低其活力水平。该试验条件下,浓度为0.01μmol/L的油菜素内酯引发24 h是缓解燕麦种子老化的最佳处理。[Objective]The aim of the present study was to explore the effects of exogenous brassinosteroids(BR)priming on aged oat(Avena sativa)seed vigor.[Method]Artificially aged oat seeds were used as experimental materials and were primed with different concentrations(0,0.01,0.10,0.50,1.00,and 10.00μmol/L)of BR solution for 0(CK),1,6,12,18,and 24 h,respectively.The changes in the germination percentage,germination index,mean germination time,and seedling vigor index were characterized.[Result]Different concentrations of exogenous BR,priming time and their interaction had extremely significant(P<0.01)effects on germination percentage,germination index,mean germination time,and seedling vigor index of the aged oat seeds.The aged oat seeds exhibited the highest germination rate,germination index,seedling vigor index as well as the lowest mean germination time when they were primed with BR at the concentration of 0.01μmol/L for 24 h.After BR priming for 1-24 h at the concentration of 10.00μmol/L,the germination rate,germination index,and seedling vigor index of the aged oat seeds were all lower than the other concentrations,while the mean germination time was significantly(P<0.05)higher than the other concentrations.[Conclusion]Low concentration of exogenous BR priming could increase the aged oat seed vigor,while high concentration exhibited the opposite effect.Under the experimental conditions in this study,the optimal treatment to alleviate the oat seed aging was priming with BR for 24 h at a concentration of 0.01μmol/L.

关 键 词：油菜素内酯 种子老化 燕麦 种子活力 种子萌发 

分 类 号：S543.704.1[农业科学—作物学]