基于非靶向代谢组学的苍耳不同药用部位代谢产物特征分析  

Analysis of Metabolite Characteristics in Different Medicinal Parts of Xanthium sibiricum Patr.Based on Non-targeted Metabolomics

作　　者：唐美琼[1] 蓝祖栽[1] 胡营[1] 韦筱媚[1] 韦桂丽 梁莹[1] 韦范[1] 杨晓男 秦双双[1] 缪剑华[1] TANG Mei-qiong;LAN Zu-zai;HU Ying;WEI Xiao-mei;WEI Gui-li;LIANG Ying;WEI Fan;YANG Xiao-nan;QIN Shuang-shuang;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园、广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023

出　　处：《中国现代中药》2023年第7期1529-1534,共6页Modern Chinese Medicine

基　　金：广西创新驱动发展专项(桂科AA18242040);对发展中国家科技援助项目(KY201904001);科技基础资源调查专项(2018FY100700);广西农业科技创新联盟项目(桂农科盟202211)。

摘　　要：目的:分析苍耳不同药用部位之间代谢产物的差异。方法:采用超高效液相色谱-电喷雾离子源-质谱法(UPLC-ESI-MS)对苍耳根、茎和叶进行测定;用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行数据处理;通过对代谢物精确相对分子质量及碎片信息的确认,结合软件数据库搜索鉴定代谢物;对差异代谢物进行京都基因与基因组百科全书(KEGG)通路富集分析。结果:苍耳的根、茎和叶的化学组成得到有效区分,从3个部位中共鉴定出5902个代谢物,筛选出64个差异代谢物,主要富集在植物次生代谢物质的生物合成,癌症中的中央碳代谢,鸟氨酸、赖氨酸和烟酸生物合成生物碱等通路。结论:从苍耳根、茎和叶代谢产物差异的角度揭示不同部位药性、药效差异的物质基础,为苍耳类中药资源的最大化综合利用提供参考。Objective:To analyze the difference of metabolites from different medicinal parts of Xanthium sibiricum Patr..Methods:The samples of root,stem,and leaf of X.sibiricum were determined by ultra-high performance liquid chromatography-electron spray ionization-mass spectrometry(UPLC-ESI-MS).Principal component analysis(PCA)and partial least-squares discriminant analysis(PLS-DA)were used for data processing.The components were identified by confirming the accurate relative molecular weight of metabolites and fragmentation information,combined with the software database search.Different metabolites were analyzed for enrichment of Kyoto encyclopedia of genes and genomes(KEGG)pathways.Results:The chemical components in root,stem,and leaf samples of X.sibiricum were clearly distinguished.A total of 5902 metabolites were identified from the above samples,and 64 different metabolites were screened,which were mainly enriched in the biosynthesis of plant secondary metabolites,central carbon metabolism in cancer,and the biosynthesis of alkaloids from ornithine,lysine,and niacin.Conclusion:From the perspective of the different metabolites in the root,stem,and leaf of X.sibiricum,the study can provide a material basis for revealing the property and efficacy in different parts of X.sibiricum and lay the foundation for the maximum comprehensive utilization of traditional medicinal resources of X.sibiricum.

关 键 词：苍耳草 非靶向代谢组学 药用部位 化学成分 代谢通路 

分 类 号：R284[医药卫生—中药学]