仙鹤草茎与叶的代谢组学比较分析  

Metabolomic Comparison between Stem and Leaf of Agrimonia pilosa Ledeb.

作　　者：万斯 杨晓男 胡营[1] 刘寒 韦坤华[1] 缪剑华[1] WAN Si;YANG Xiao-nan;HU Ying;LIU Han;WEI Kun-hua;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园、广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023

出　　处：《中国现代中药》2023年第7期1599-1610,共12页Modern Chinese Medicine

基　　金：广西创新驱动发展专项(桂科AA18242040);对发展中国家科技援助项目(KY201904001);广西农业科技创新联盟项目(桂农科盟202211)。

摘　　要：目的:通过代谢组学分析仙鹤草茎与叶中代谢物及其代谢通路差异,进一步阐明仙鹤草药效物质基础,促进仙鹤草合理开发利用。方法:取仙鹤草茎、叶新鲜样本,经液氮冷冻后提取代谢物,采用液相色谱-质谱法检测,所得数据运用统计学方法分析并与数据库比对鉴定。结果:筛选并鉴定出105个具有明显差异的代谢物,其中茎与叶相比有34个代谢物上调、71个代谢物下调。差异代谢物共注释到62条代谢通路中。茎中叶黄素、天冬酰胺、胍丁胺及多种氨基酸等物质含量更高,氨基酸代谢更强;叶中酚酸类、黄酮类物质积累更为丰富,次生代谢更强。结论:仙鹤草茎与叶均含有丰富的药效活性成分,但叶的次生代谢更强,次生代谢物积累更为丰富,此差异或可为仙鹤草针对性开发利用提供参考。Objective:To compare the metabolites and metabolic pathways between the stems and leaves of Agrimonia pilosa Ledeb.by metabolomics and further clarify the pharmacodynamic substances and promote the rational development and utilization of A.pilosa.Methods:Fresh stem and leaf samples of A.pilosa were frozen with liquid nitrogen,and then the metabolites were extracted and detected by liquid chromatography-mass spectrometry.The obtained data were analyzed by statistical methods and compared with those in database.Results:A total of 105 differential metabolites were identified,including 34 up-regulated metabolites and 71 down-regulated metabolites in the stems compared with those in the leaves.The differential metabolites were annotated into 62 metabolic pathways.The stems showed higher content of lutein,L-asparagine,agmatine,and a variety of amino acids and stronger amino acid metabolism.The leaves had higher accumulation of phenolic acids and flavonoids and more active secondary metabolism.Conclusion:Both stems and leaves of A.pilosa contained abundant pharmacodynamic components,and the leaves demonstrated more active secondary metabolism and contained more abundant secondary metabolites.These differences may provide reference for the targeted development and utilization of the herb.

关 键 词：仙鹤草 代谢组学 差异代谢物 代谢通路 

分 类 号：R284[医药卫生—中药学]