基于非靶向代谢组学的蜘蛛香不同部位差异代谢物分析  

Analysis of Different Metabolites in Different Parts of Valeriana jatamansi Jones Based on Non-targeted Metabolomics

作　　者：胡营[1] 俞巧玲 郭晓云[1] 韦筱媚[1] 韦坤华[1,2] 缪剑华 HU Ying;YU Qiao-ling;GUO Xiao-yun;WEI Xiao-mei;WEI Kun-hua;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;School of Pharmacy,Guangxi Medical University,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园、广西药用资源保护与遗传改良重点实验室/广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023 [2]广西医科大学药学院,广西南宁530023

出　　处：《中国现代中药》2023年第7期1619-1626,共8页Modern Chinese Medicine

基　　金：广西创新驱动发展专项(桂科AA18242040);对发展中国家科技援助项目(KY201904001);科技基础资源调查专项(2018FY100700);广西农业科技创新联盟项目(桂农科盟202211);广西科技基地与人才专项(桂科AD22080012)。

摘　　要：目的:研究蜘蛛香不同部位的代谢物差异,促进蜘蛛香资源的综合利用。方法:利用非靶向代谢组学技术,对蜘蛛香的根茎、根和叶3个部位的代谢物进行差异性比较分析。结果:从蜘蛛香的根茎、根和叶中共鉴定出491个代谢物,主要代谢物有挥发油、多糖、氨基酸等。蜘蛛香根茎、根和叶中的代谢物差异显著,根与叶、根茎与叶、根与根茎之间的差异代谢物分别为145、109、122个,根茎和叶中含莪术烯醇、4-羟基肉桂酸、芥子酸胆碱、5-羟基-1-四氢萘酮、4-乙基苯甲醛、松脂醇4-O-β-D-吡喃葡萄糖苷和5-胸苷酸二钠盐较少,而根中较多;根和根茎中含茶碱、芹菜素和阿尔泊酚酸较少,而叶中较多。根茎、根和叶的差异代谢物主要富集在类苯丙酸生物合成通路,氨酰tRNA生物合成通路和丙氨酸、天冬氨酸与谷氨酸代谢通路。结论:蜘蛛香的根茎、根和叶中含有挥发油、多糖、氨基酸等大量的差异代谢物,研究结果可为蜘蛛香不同部位关键代谢物的挖掘和充分利用提供参考。Objective:To improve the comprehensive utilization of Valeriana jatamansi Jones resources by analyzing the different metabolites in different parts of V.jatamansi.Methods:The differences in metabolites in the rhizome,root,and leaf of V.jatamansi were studied by using non-targeted metabolomics.Results:A total of 491 metabolites were identified from the rhizome,root,and leaf of V.jatamans,and the main metabolites were volatile oil,polysaccharide,amino acid,etc.The metabolites in the rhizome,root,and leaf of V.jatamansi were significantly different.The different metabolites between root and leaf,rhizome and leaf,and root and leaf were 145,109,and 122,respectively.The curcumol,4-hydroxycinnamic acid,mustard acid choline,5-hydroxy-1-tetrahydronaphthone,4-ethyl benzoaldehyde,terpineol 4-O-β-Dglucopyranoside,and 5-thymidylate disodium salt were less in rhizome and leaf but more in the root.There were less theophylline,apigenin,and alphenolic acid in the root and rhizome but more in the leaf.The different metabolites of the rhizome,root,and leaf were mainly concentrated in the phenylalanine biosynthesis pathway,aminoacyl tRNA biosynthesis pathway,and alanine,aspartic acid,and glutamate metabolism pathway.Conclusion:Volatile oil,polysaccharide,amino acid,and other different metabolites were founded in the rhizome,root,and leaf of V.jatamansi.This study provides a reference for exploring and fully utilizing key metabolites in different parts of V.jatamansi.

关 键 词：蜘蛛香 非靶向代谢组学 差异代谢物 通路分析 

分 类 号：R284[医药卫生—中药学]