基于全基因组重测序技术的甘蓝型油菜光叶突变体基因定位  

Gene mapping of a novel glossy mutant in Brassica napus L.based on whole genome resequencing technology

作　　者：文雁成[1] 何俊平[1] 蔡东芳[1] 张书芬[1] 朱家成[1] 王建平[1] 曹金华[1] 赵磊[1] 王东国[1] 刘奕孜[1] WEN Yancheng;HE Junping;CAI Dongfang;ZHANG Shufen;ZHU Jiacheng;WANG Jianping;CAO Jinhua;ZHAO Lei;WANG Dongguo;LIU Yizi(Institute of Industrial Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《浙江大学学报（农业与生命科学版）》2023年第4期497-506,共10页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：“十三五”国家重点研发计划重点专项(2016YFD0101300);国家现代农业(油菜)产业技术体系建设专项(CARS-12)。

摘　　要：表皮蜡质是油菜适应逆境的保护措施之一。本课题组前期报道了一个由1对显性基因控制的甘蓝型油菜(Brassica napus L.)光叶突变体DL22B077-1。为了进一步了解其遗传机制,本研究利用全基因组重测序技术和分离群体分析法(bulk segregated analysis,BSA)通过F2群体进行基因定位,获得的有效碱基数达10661.75 Mb,其中,Q30均值为92.99%,平均作图率为97.73%,平均测序深度为24×,平均覆盖率为82.06%,测序质量较高;此外,分析了单核苷酸多态性(single nucleotide polymorphism,SNP)标记和插入-缺失(insertion-deletion,In-Del)标记与光叶性状的相关性。通过SNP标记,在5条染色体上得到7个相关区域和1509个相关基因。通过In-Del标记,在11条染色体上得到15个相关区域和2633个相关基因。SNP标记和In-Del标记关联分析结果的交集部分为C8染色体上8.60~10.39 Mb区域,总长度为1.79 Mb,共计130个候选基因,其中119个基因得到注释,它们参与了细胞成分的构建,确保了分子功能和生物过程的顺利进行。DL22B077-1是第一个将蜡质基因定位到C8染色体上的甘蓝型油菜光叶突变体,据此判断其为新的光叶突变体。上述结果为选育高产稳产油菜品种、改良油菜抗逆栽培技术奠定了理论基础。Epicuticular wax in the rape is one of the protective barriers in stress environments.We previously reported one glossy mutant DL22B077-1 in Brassica napus L.controlled by a pair of dominant genes.In order to further understand its genetic mechanism,the glossy genes in the F_(2)population were mapped by whole genome resequencing technology and bulk segregated analysis(BSA)in this study.A total of 10661.75 Mb of clean bases were obtained,with an average Q30 of 92.99%,an average mapping ratio of 97.73%,an average sequencing depth of 24×,and an average coverage ratio of 82.06%,which indicated that the sequencing quality was high.In addition,the correlations between the glossy characters and markers of single nucleotide polymorphism(SNP)and insertion-deletion(In-Del)in genomic domains were analyzed.In the SNP marker,seven associated regions and 1509 genes on five chromosomes were obtained.In the In-Del marker,15 associated regions and 2633 genes on 11 chromosomes were obtained.The final association results were the intersection of two kinds of markers,which was the 1.79 Mb associated region from 8.60 Mb to 10.39 Mb on chromosome C8,and there were a total of 130 candidate genes in this region.A total of 119 genes in the 130 candidate genes were annotated,which participated in the construction of cellular components and were involved in molecular functions and biological processes.In B.napus,DL22B077-1 was the first glossy mutant whose glossy genes were located on chromosome C8,so it was a novel glossy mutant.The above results lay a theoretical foundation for breeding rape varieties with high and stable yields and improving resistance cultivation techniques.

关 键 词：甘蓝型油菜 光叶突变体 全基因组重测序 基因定位 

分 类 号：S565.4[农业科学—作物学] Q343.17[生物学—遗传学]