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作 者:古昌辉 许明珠[2] 程光平 程蔓 韦云勇 麻艳群 GU Changhui;XU Mingzhu;CHENG Guangping;CHENG Man;WEI Yunyong;MA Yanqun(College of Animal Science and Technology/Key Laboratory of Healthy Breeding and Nutrition Regulation of Aquatic Organisms in Guangxi Universities,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Institute of Oceanology Co.,LTD.,Beihai,Guangxi 536000,China)
机构地区:[1]广西大学动物科学技术学院/广西高校水生生物健康养殖与营养调控重点实验室,广西南宁530004 [2]广西海洋研究所有限责任公司,广西北海536000
出 处:《水产养殖》2023年第8期29-35,共7页Journal of Aquaculture
基 金:(巴马)油鱼规模化人工繁殖集成技术研究(桂科AB18221116)。
摘 要:设置4个试验组(A、B、C、D)和1个对照组(E),在不同的试验条件下,对巴马拟缨鱼进行人工催产,采用双抗体夹心法,测定样本鱼催产前后血清激素水平,试验周期365 d。结果表明,催产前,C组的雌二醇(E_(2))含量最高,较A组有显著提高(P<0.05),与其他各组无显著差异(P>0.05);D组的雌三醇(E_(3))和睾酮(T)含量最高,各组间无显著差异(P>0.05);A组的孕酮(P)含量最高,组间差异不显著(P>0.05);B组雌酮(E_(1))含量最高,较A组和D组有显著提高(P<0.05),与C组无显著差异(P>0.05)。催产后,A组E2含量上升,B、C、D组下降,且C组催产前后差异显著(P<0.05);A、B组E_(3)含量均上升,其中B组催产前后差异显著(P<0.05),C、D组下降,催产前后差异不显著;A、B组P含量下降,而C、D组上升,催产前后差异不显著(P>0.05);A、B组E_(1)含量下降,A组催产前后差异显著(P<0.05),B组催产前后无显著差异(P>0.05),C、D组上升,催产前后差异显著(P<0.05)。指出,B、C和D组有助于提高巴马拟缨鱼E_(1)、E_(2)、E_(3)和P水平,进而促进人工培育条件下的性腺发育,对催产激素有较高的敏感性,是培育巴马拟缨鱼亲本的可选模式。Four experimental groups(A,B,C,D)and one control group(E)were set up to artificially induce production of Bama tama under different experimental conditions,The serum hormone level of the sample fish before and after labor was measured by the double antibody sandwich method of sampling and the test period was 365 days.The results showed that before induction,the content of estradiol(E_(2))in group C was the highest,which was significantly higher than that in group A(P<0.05),and there was no significant difference with other groups(P>0.05);The content of estriol(E_(3))and testosterone(T)was highest in group D,with no significant difference between groups(P>0.05);The content of progesterone(P)was the highest in group A,with no significant difference between groups(P>0.05),and the content of estrone(E_(1))in group B was the highest,which was significantly higher than that in group A and group D(P<0.05),and there was no significant difference with group C(P>0.05);After induction,the content of E_(2) in group A increased,and the B,C and D groups decreased,and the difference before and after labor induction in group C was significant(P<0.05);the content of E_(3) in group A and B increased,among which the difference before and after labor induction in group B was significant(P<0.05),and the difference before and after labor induction decreased in group C and D,and the difference before and after labor induction was not significant.The P content of groups A and B decreased,while the P content in groups C and D increased,and the difference before and after labor induction was not significant.The content of E_(1) decreased in groups A and B,and there were significant differences before and after labor induction in group A(P<0.05),there was no significant difference before and after labor induction in group B(P>0.05),and groups C and D increased,and the difference before and after labor induction was significant(P<0.05).It was pointed out that groups B,C and D were helpful in increasing the levels of E_(1),E_(2),E_(3) and P
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