木霉菌配施有机肥对甜瓜果实糖及代谢酶活性的影响  

Effects of Trichoderma Combined with Organic Fertilizer on the Sugar Contents and Glucose Metabolic Enzymes Activities in Muskmelon

作　　者：李春霞[1] 聂佳慧 陈钱广 高佳怡 肖漫 王雨舒 熊亚男 靳亚忠[1] Li Chunxia;Nie Jiahui;Chen Qianguang;Gao Jiayi;Xiao Man;Wang Yushu;Xiong Ya’nan;Jin Yazhong(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学园艺园林学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2023年第4期19-25,共7页journal of heilongjiang bayi agricultural university

基　　金：大庆市指导性科技计划项目(zd-2021-83);露地蔬菜化肥农药减施技术集成研究与示范-东北寒区露地蔬菜化肥农药减施技术模式建立与示范(2018YFD0201206);黑龙江八一农垦大学三纵项目(ZRCPY202116)。

摘　　要：研究以提高甜瓜品质,降低化肥的施用量为目的,通过大田试验,探究减施化肥配施有机肥和木霉菌剂对甜瓜果实糖及代谢酶活性的影响,为优质甜瓜栽培提供理论参考。以“CH7”薄皮甜瓜为研究对象,设置5个处理,分析果实糖分积累以及蔗糖相关代谢酶活性在生长过程中的动态变化。结果表明:与CK(不施肥处理)和CK1(农民常规施肥)相比较,减施化肥配施有机肥和木霉菌的(T1、T2、T3)处理显著增加了甜瓜可溶性糖和蔗糖含量,同时T2处理在花后35 d和T3处理在花后28 d和35 d提高了甜瓜的果糖和葡萄糖含量。与CK和CK1相比,T1、T2和T3处理增强了果实中的蔗糖合成酶-合成方向酶(SS-s)和蔗糖磷酸合成酶(SPS)活性和花后28 d甜瓜的中性转化酶活性(NI);除了T1处理在花后21 d,其它处理显著抑制了蔗糖合成酶-分解方向酶活性(SS-d);除了T3处理在花后21 d,其它处理显著抑制了酸性转化酶活性(AI)。由此可见,减施化肥配施有机肥和木霉菌,通过调节糖代谢酶之间的协调性,进而促进不同成熟时期甜瓜果实糖分的积累。In order to improve the quality of muskmelon and reduce the application amount of chemical fertilizer,field experiments were conducted to explore the effects of reducing the application of chemical fertilizer combined with organic fertilizer and trichoderma on the sugar and metabolic enzyme activities of muskmelon fruits,providing theoretical reference for the cultivation of high-quality muskmelon.Taking"CH7"thin-skinned muskmelon as the research object,five treatments were set up to analyze the dynamic changes of sugar accumulation and sucrose related metabolic enzyme activities during fruit growth.The results showed as follows:compared with CK(no fertilization)and CK1(conventional fertilization by farmers),the treatment of reducing fertilizer combined with organic fertilizer and trichoderma(T1,T2,T3)significantly increased the soluble sugar and sucrose contents of muskmelon,and the treatment of T2 and T3 increased the fructose and glucose contents of melon at 35 d after flowering and at 28 d and 35 d after flowering.Compared with CK and CK1,T1,T2 and T3 treatments increased the activities of sucrose synthetase-synthetase(SS-s)and sucrose phosphate synthetase(SPS)and neutral invertase activity(NI)at 28 days after flowering in muskmelons.Except for T1 treatment at 21 days after anthesis,other treatments significantly inhibited the activity of sucrose synthase-decomposition direction enzyme(SS-d).Except for T3 treatment at 21d after anthesis,other treatments significantly inhibited acid invertase activity(AI).In conclusion,reducing the application of chemical fertilizer combined with the application of organic fertilizer and trichoderma could promote the accumulation of sugar in melon fruits at different ripening stages by regulating the coordination of sugar metabolism enzymes.

关 键 词：薄皮甜瓜 木霉菌 糖分积累 糖代谢酶 

分 类 号：S641.3[农业科学—蔬菜学]