Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4^(+)T cells  

作　　者：Jiung Jeong In Kang Yumin Kim Keun Bon Ku Jang Hyun Park Hyun-Jin Kim Chae Won Kim Jeongwoo La Hi Eun Jung Hyeon Cheol Kim Young Joon Choi Jaeho Kim Joon Kim Heung Kyu Lee 

机构地区：[1]Graduate School of Medical Science and Engineering,Korea Advanced Institute of Science and Technology(KAIST),Daejeon 34141,Republic of Korea [2]Department of Internal Medicine,Seoul National University Hospital,Seoul 03080,Republic of Korea [3]Department of Biological Sciences,KAIST,Daejeon 34141,Republic of Korea [4]Department of Convergent Research of Emerging Virus Infection,Korea Research Institute of Chemical Technology,Daejeon 34114,Republic of Korea.

出　　处：《Cellular & Molecular Immunology》2023年第5期525-539,共15页中国免疫学杂志（英文版）

基　　金：the National Research Foundation of Korea(NRF-2021M3A9D3026428);the Ministry of Science and ICT of Korea.J.Jeong is a recipient of funding from the Global Ph.D.Fellowship Program(NRF-2019H1A2A1076865)of the National Research Foundation of Korea.

摘　　要：CD4^(+)T cells play major roles in the adaptive immune system,which requires antigen recognition,costimulation,and cytokines for its elaborate orchestration.Recent studies have provided new insight into the importance of the supramolecular activation cluster(SMAC),which comprises concentric circles and is involved in the amplification of CD4^(+)T cell activation.However,the underlying mechanism of SMAC formation remains poorly understood.Here,we performed single-cell RNA sequencing of CD4^(+)T cells left unstimulated and stimulated with anti-CD3 and anti-CD28 antibodies to identify novel proteins involved in their regulation.We found that intraflagellar transport 20(IFT20),previously known as cilia-forming protein,was upregulated in antibody-stimulated CD4^(+)T cells compared to unstimulated CD4^(+)T cells.We also found that IFT20 interacted with tumor susceptibility gene 101(TSG101),a protein that endocytoses ubiquitinated T-cell receptors.The interaction between IFT20 and TSG101 promoted SMAC formation,which led to amplification of AKT-mTOR signaling.However,IFT20-deficient CD4^(+)T cells showed SMAC malformation,resulting in reduced CD4^(+)T cell proliferation,aerobic glycolysis,and cellular respiration.Finally,mice with T-cell-specific IFT20 deficiency exhibited reduced allergen-induced airway inflammation.Thus,our data suggest that the IFT20-TSG101 axis regulates AKT-mTOR signaling via SMAC formation.

关 键 词：CD4^(+)T cell Intraflagellar transport 20 Tumor susceptibility gene 101 AKT-mTOR signaling Asthma Supramolecular activation cluster 

分 类 号：R392[医药卫生—免疫学]