The domestication-associated L1 gene encodes a eucomic acid synthase pleiotropically modulating pod pigmentation and shattering in soybean  

作　　者：Xiangguang Lyu Ying-hui Li Yanfei Li Delin Li Chao Han Huilong Hong Yu Tian Lida Han Bin Liu Li-juan Qiu 

机构地区：[1]The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing,P.R.China [2]Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing,P.R.China [3]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Bejing,P.R.China [4]Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing,P.R.China

出　　处：《Molecular Plant》2023年第7期1178-1191,共14页分子植物（英文版）

基　　金：the National Key Research and Development Program of China(2021YFD1201601,2021YFF1001201);the earmarked fund for CARS(CARS-04-PSO1);the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences;the National Natural Science Foundation of China(grant no.32201734);the China Postdoctoral Science Foundation(grant no.2021M693465).

摘　　要：Pod coloration is a domestication-related trait in soybean,with modern cultivars typically displaying brown or tan pods,while their wild relative,Glycine soja,possesses black pods.However,the factors regulating this color variation remain unknown.In this study,we cloned and characterized L1,the classical locus responsible for black pods in soybean.By using map-based cloning and genetic analyses,we identified the causal gene of L1 and revealed that it encodes a hydroxymethylglutaryl-coenzyme A(CoA)lyase-like(HMGL-like)domain protein.Biochemical assays showed that L1 functions as a eucomic acid synthase and facilitates the synthesis of eucomic acid and piscidic acid,both of which contribute to coloration of pods and seed coats in soybean.Interestingly,we found that L1 plants are more prone to pod shattering under light exposure than l1 null mutants because dark pigmentation increases photothermal efficiency.Hence,pleiotropic effects of L1 on pod color and shattering,as well as seed pigmentation,likely contributed to the preference forl1 alleles during soybean domestication and improvement.Collectively,our study provides new insights into the mechanism of pod coloration and identifies a new target for future de novo domestication oflegume crops.

关 键 词：SOYBEAN DOMESTICATION pod pigmentation pod shattering pleiotropic effects 

分 类 号：S643.7[农业科学—蔬菜学]