喷施BABA调控枸杞活性氧代谢提高果实采后抗病能力  

Spraying BABA Regulate Reactive Oxygens Metabolism of Wolfberry Fruits to Improve Disease Resistance of Postharvest

作　　者：冯丽丹[1] 李捷[2,3] 何静 李永晖[2] 张煦 刘祥林 FENG Lidan;LI Jie;HE Jing;LI Yonghui;ZHANG Xu;LIU Xianglin(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou,Gansu 730070;College of Forestry,Gansu Agricultural University,Lanzhou,Gansu 730070;Gansu Wolfberry Harmless Cultivation Engineering Research Center,Lanzhou,Gansu 730070)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃农业大学林学院,甘肃兰州730070 [3]甘肃省枸杞无害化栽培工程研究中心,甘肃兰州730070

出　　处：《北方园艺》2023年第13期112-120,共9页Northern Horticulture

基　　金：甘肃省高校创新基金资助项目(2020A-055);甘肃省特色优势农产品评价资助项目(TYNPZ2022-13)。

摘　　要：以六年生枸杞‘宁杞7号’植株为试材,采用叶面喷施β氨基丁酸(BABA)的方法,研究了BABA对枸杞果实失重率、自然发病率、MDA含量、主要活性氧、抗氧化酶和抗氧化物质的影响,以期为增强枸杞鲜果采后抗病能力,减少果实的腐烂,延长保鲜时间提供参考依据。结果表明:采前叶面喷施1.0 mg·m^(-1)BABA诱导21 d后,可明显降低采后枸杞果实的失重率和自然腐烂率;抑制果实中超氧阴离子(O·_(2)^(-))和过氧化氢(H_(2)O_(2))的产生,显著提升超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活性;提升抗坏血酸-谷胱甘肽(AsA-GSH)循环中抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)的活性,提高抗坏血酸(AsA)和脱氧抗坏血酸还原酶(DHA)等非酶促抗氧化剂的含量,降低丙二醛(MDA)含量。在枸杞第1茬坐果后开始施用1 mg·mL^(-1)BABA,每7 d施用1次,可通过激活枸杞果实抗氧化酶活性,提升非酶促抗氧化剂的含量,将果实氧化程度控制在较低水平,有效减少果实的腐烂,延长保鲜时间。The six years old wolfberry‘Ningqi VII’was used as the test material,and foliar spraying theβ-Aminobutyric acid(BABA).The weight loss rate,natural incidence rate,MDA content,main active oxygen species,antioxidant enzymes and antioxidant substances effect by BABA in the wolfberry fruit were studied,in order to provide reference of wolfberry fresh fruit for enhancing the disease resistance after harvest,reducing fruit decay,and extending the preservation time.The results showed that foliar spraying 1.0 mg·mL^(-1)BABA before harvest,and induced 21 days could significantly reduce the weight loss rate and natural decay rate of postharvest wolfberry fruits;inhibited the production of O·_(2)^(-)and H_(2)O_(2)in fruits;increased the activities of antioxidant enzymes such as SOD,POD and CAT significantly;improved the activity of APX and GR,increased the content of non enzymatic antioxidants such as ASA and DHA in ASAGSH cycle;and reduced the content of MDA.Therefore,after the first fruit setting of wolfberry foliar sprayed 1.0 mg·mL^(-1)BABA for every 7 days,which could increase the content of non enzymatic antioxidants and activate the activity of antioxidant enzyme,control the degree of oxidation at a lower level,reduce the decay of fruit effectively,and extend the freshness preservation time.

关 键 词：Β-氨基丁酸 枸杞 抗病能力 活性氧 抗氧化 

分 类 号：S567.19[农业科学—中草药栽培]