湿加松个体老化模式与平茬复幼的机理  

The ontogenetic ageing pattern and the molecular mechanism for prunning rejuvenation in Pinus elliottii×P.caribaea

作　　者：杨舜垚 张贵芳 张曦[1,2,3] 林昌明 黄婷 邓乐平 张智霖 李福明 钟岁英 盘晓莺 何其邹洪 张莹莹 鄂一岚 沈微微[1,2,3] 王广超 李洋 赵媛媛 李晓娟 黄少伟 沈应柏[1,2,3] 钮世辉[1,2,3] 林金星 YANG ShunYao;ZHANG GuiFang;ZHANG Xi;LIN ChangMing;HUANG Ting;DENG LePing;ZHANG ZhiLin;LI FuMing;ZHONG SuiYing;PAN XiaoYing;HE QiZouHong;ZHANG YingYing;E YiLan;SHEN WeiWei;WANG GuangChao;LI Yang;ZHAO YuanYuan;LI XiaoJuan;HUANG ShaoWei;SHEN YingBai;NIU ShiHui;LIN JinXing(National Engineering Research Center of Tree Breeding and Ecological Restoration,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China;Institute of Tree Development and Genome Editing,Beijing Forestry University,Beijing 100083,China;Guangdong Hongling Seed Garden,Taishan 529200,China;China National Tree Seed Group Corporation Limited,Beijing 100036,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,Guangzhou 510642,China)

机构地区：[1]北京林业大学生物科学与技术学院,林木育种与生态修复国家工程研究中心,北京100083 [2]北京林业大学生物科学与技术学院,林木、花卉遗传育种教育部重点实验室,北京100083 [3]北京林业大学,树木发育与基因编辑研究院,北京100083 [4]广东省台山市红岭种子园,台山529200 [5]中国林木种子集团有限公司,北京100036 [6]华南农业大学林学与风景园林学院,广州510642 [7]广东省森林植物种质创新与利用重点实验室,广州510642

出　　处：《中国科学：生命科学》2023年第8期1146-1165,共20页Scientia Sinica(Vitae)

基　　金：国家自然科学基金项目(批准号:32030010,91954202,32001293,32000558);北京林业大学优秀研究生导师团队建设项目(批准号:YJSYDSTD2022005);北京林业大学“杰出青年人才”培育计划(批准号:2019JQ03003);中央高校基本科研业务费专项资金(批准号:BLX202116);科技创新2030(批准号:2022ZD0401605)资助。

摘　　要：松树是我国重要的针叶树造林树种.在早期的生产实践中,通过树木复幼等技术,可以有效提升多种针叶树树种的无性繁育,但缺乏从分子水平上对其进行评价和测算.本研究以实生湿加松和平茬复幼的湿加松(Pinus elliottii×P.caribaea)为研究对象,结合转录组测序、加权基因共表达网络分析、荧光实时定量PCR等多种技术,鉴定到Pt6G14330,Pt6G55590,Pt9G03170,Pt3G66280(AP2L3),PtJG21230,Pt4G16540,Pt2G41770(DAL10),Pt1G04760(MADS28)等年龄标记基因,同时借助这些基因阐明了湿加松个体发育老化的空间模式,并揭示了平茬复幼的分子机制.本研究还发现,平茬后湿加松嫩枝中茉莉素、水杨酸、生长素等含量显著上升,脱落酸含量显著下降,暗示树木的平茬复幼与激素变化密切相关.这一研究不仅从分子水平厘清了针叶树的组织年龄变化规律,也为树木高效规模化繁育过程中复合生长调节剂的研发提供了理论指导.Pine is an important coniferous tree for afforestation in China.During the early production practice,the clone reproduction of several coniferous species was successfully performed based on rejuvenation techniques,which were not evaluated and measured at the molecular level yet.In this study,the seedlings and prunned seedlings of Pinus elliottii×P.caribaea were used as research materials,and age-marking genes including Pt6G14330,Pt6G55590,Pt9G03170,Pt3G66280(AP2L3),PtJG21230,Pt4G16540,Pt2G41770(DAL10),Pt1G04760(MADS28)were identified by transcriptome sequencing,Weighted gene co-expression network analysis,and quantitative real-time PCR.In addition,these genes were also used to elucidate the spatial patterns of ontogenetic ageing of Pinus elliottii×P.caribaea and to reveal the molecular mechanism of prunning rejuvenation.Moreover,we also found that the content of jasmonate,salicylic acid,and auxin in the juvenile shoots of Pinus elliottii×P.caribaea significantly increased after prunning,whereas the content of abscisic acid decreased significantly,suggesting that the rejuvenation by prunning was closely related to the change of endogenous hormones.This study clarifies the regulatory mechanism of the age variation in organs of coniferous species at the molecular level and provides theoretical guidance for the preparation of plant growth regulators in the large-scale breeding of trees.

关 键 词：湿加松 个体发育老化 平茬 复幼 分子证据 规模化繁育 

分 类 号：S791.24[农业科学—林木遗传育种]