抗核抗体与抗可提取性核抗原抗体联合检测对自身免疫疾病的诊断价值  

Diagnostic value of anti-nuclear antibodies combined with anti-extractable nuclear antigen antibodies in autoimmune diseases

作　　者：徐细兰 严良烽 谢超 吴辉云 XU Xilan;YAN Liangfeng;XIE Chao;WU Huiyun(Department of Laboratory Medicine,Jingdezhen NO.1 People's Hospital,Jingdezhen,Jiangxi,333000,China)

机构地区：[1]景德镇市第一人民医院检验科,江西景德镇333000

出　　处：《当代医学》2023年第6期38-42,共5页Contemporary Medicine

基　　金：景德镇市科技计划项目(20201SFZC016)。

摘　　要：目的探讨抗核抗体(ANA)与抗可提取性核抗原(ENA)抗体联合检测对自身免疫疾病的诊断价值。方法选取2018年1月至2020年9月本院收治的63例自身免疫疾病患者作为研究组,另选取同期本院60名健康体检者作为对照组,两组均进行ANA、抗ENA抗体检测。比较两组ANA阳性率,分析两组抗ENA抗体检测结果,比较研究组不同自身免疫疾病患者抗ENA抗体阳性率,分析ANA联合抗ENA抗体在自身免疫疾病中的诊断效能。结果研究组SLE、RA、SS患者ANA阳性率均高于对照组,且SLE患者高于RA及SS患者,SS患者高于RA患者,差异有统计意义(P<0.05)。研究组SLE、RA、SS患者均不同程度检出抗ENA抗体阳性,对照组抗ENA抗体检测均为阴性;研究组SLE、RA、SS患者RNP、Sm、SS-A、SS-B、Scl-70阳性率比较差异有统计学意义(P<0.05),SLE、RA、SS患者Jo-l阳性率比较差异无统计学意义;两两比较:研究组SLE患者RNP、Sm、SS-A、SS-B阳性率均高于RA患者,差异有统计学意义(P<0.05),SLE患者与RA患者Scl-70、Jo-l阳性率比较差异无统计学意义;SLE患者RNP阳性率高于SS患者,Scl-70阳性率低于SS患者(P<0.05),SLE患者与SS患者Sm、SS-A、SS-B、Jol阳性率比较差异无统计学意义;RA患者SS-A、SS-B阳性率均低于SS患者,差异有统计学意义(P<0.05),RA患者与SS患者RNP、Sm、Scl-70、Jo-l阳性率比较差异无统计学意义。绘制ROC曲线结果显示,ANA联合抗ENA抗体检测诊断自身免疫疾病的AUC为0.875,诊断价值较高;ANA联合抗ENA抗体检测诊断自身免疫疾病的灵敏度、特异度均高于ANA和抗ENA抗体单一检测(P<0.05),ANA与抗ENA抗体单一检测诊断自身免疫疾病的灵敏度、特异度比较差异无统计学意义。结论ANA与抗ENA抗体联合检测应用于自身免疫疾病患者中,可提高不同类型的自身免疫疾病自身抗体阳性检出率,具有较高的诊断价值,可为临床制订治疗方案提供准确的指导。Objective To explore the diagnostic value of anti-nuclear antibodies(ANA)combined with anti-extractable nuclear antigen antibod-ies(ENA)in autoimmune diseases.Methods 63 patients with autoimmune diseases admitted to our hospital from January 2018 to September 2020 were selected as the research group,and 60 healthy physical examination subjects in our hospital during the same period were selected as the control group,ANA and anti-ENA antibody were detected in the two groups.The positive rate of ANA between the two groups was compared,the detection results of anti-ENA antibody between the two groups were analyzed,the positive rate of anti-ENA antibody in patients with different autoimmune diseases in the research group were compared,and the diagnostic efficacy of ANA combined with anti-ENA antibody in autoimmune diseases was analyzed.Results The positive rate of ANA in SLE,RA and SS patients in the research group was higher than that in the control group,and SLE patients was higher than RA and SS patients,and SS patients was higher than that in RA patients,the differences were statistically significant(P<0.05).Anti-ENA antibody was positive in SLE,RA and SS patients in the research group,and negative in the control group;there were statistically significant differences in the positive rates of RNP,Sm,SS-A,SS-B and Scl-70 in SLE,RA and SS patients in the research group(P<0.05),while there were no statistically significant differences in the positive rates of Jo-l in SLE,RA and SS patients;pairwise comparison:the positive rates of RNP,Sm,SS-A and SS-B in SLE patients were higher than those in RA patients in the research group,and the differences were statistically significant(P<0.05);but there was no statistically significant difference in the positive rates of Scl-70 and Jo-l between SLE patients and RA patients;the positive rate of RNP in SLE patients was higher than that in SS patients,and the positive rate of Scl-70 was lower than that in SS patients(P<0.05);there was no statistical significance in the positive r

关 键 词：自身免疫疾病 抗核抗体 抗可提取性核抗原抗体 联合检测 诊断价值 

分 类 号：R446.6[医药卫生—诊断学] R593.2[医药卫生—临床医学]