黄柄壁泥蜂共生链霉菌R1702-2发酵产物细胞毒性研究  

Cytostatic activity of fermentation products from symbiotic Streptomyces sp.R1702-2 associated with Sceliphron madraspatanum

作　　者：张修梅 谢卫东[2] 陆丹丹 李世伟 李振兴 王艳红 邹小丽 ZHANG Xiu-mei;XIE Wei-dong;LU Dan-dan;LI Shi-wei;LI Zhen-xing;WANG Yan-hong;ZOU Xiao-li(Shandong Drug and Food Vocational College,Weihai 264210,China;School of Marine Science,Shandong University,Weihai 264209,China)

机构地区：[1]山东药品食品职业学院,山东威海264210 [2]山东大学海洋学院,山东威海264209

出　　处：《化学研究与应用》2023年第8期1939-1944,共6页Chemical Research and Application

基　　金：山东省自然科学基金项目(ZR2014HM018)资助。

摘　　要：运用硅胶柱层析和半制备型高效液相等分离方法从黄柄壁泥蜂共生链霉菌R1702-2的发酵产物中进行分离纯化,得到5个单体化合物。通过解析核磁数据及相关文献对比,确定化合物为collinone(1)、γ-rubromy-cin(2)、δ-rubromycin(3)、β-rubromycin(4)和3′-hydroxy-β-rubromycin(5)。Streptomyces sp.R1702-2是一个产rubromycins家族抗生素的新菌株。利用MTT法测定这5个化合物对肿瘤细胞及正常细胞系的细胞毒性,结果表明化合物3、4、5对于肺癌细胞株A549有较好的细胞毒活性,IC_(50)值分别为9.304、9.158和5.723 uM。Five compounds,collinone(1),γ-rubromycin(2),δ-rubromycin(3),β-rubromycin(4)and 3′-hydroxy-β-rubromycin(5),were isolated by silica gel column chromatography and semi-HPLC from symbiotic Streptomyces sp.R1702-2 associated with Sceliphron madraspatanum.Their structures were determined by NMR data and comparison with literature data.Streptomyces R17022 was a new rubromycin-producing srain.Cytostatic activity against different tumor cell and normal cell lines was determined by MTT assay.Compounds 3,4 and 5 possessed a marked cytostatic activity against A549(lung carcinoma),with IC_(50)values of 9.304,9.158 and 5.723 uM,respectively.

关 键 词：黄柄壁泥蜂 链霉菌 发酵产物 分离鉴定 细胞毒性 

分 类 号：O657[理学—分析化学]