蒙农杂种冰草AcdMYB 1基因克隆及表达分析  

Cloning and Expression Analysis of the AcdMYB 1 Gene from Agropyron critatum×A.desertorum‘Hycrest-Mengnong’

作　　者：黄馨田 韩慧杰 李宇琛 刘亚玲 张雅荣 赵彦 HUANG Xin-tian;HAN Hui-jie;LI Yu-chen;LIU Ya-ling;ZHANG Ya-rong;ZHAO Yan(College of Grassland,Resources and Environmental Science,Inner Mongolia Agricultural University/Key Laboratory of Forage Cultivation,Processing and High Efficient Utilization of Ministry of Agriculture/Key Laboratory of Grassland Resources,Ministry of Education,Hohhot,Inner Mongolia 010018,China;MenCao Ecology and Environment(Group)Co.,Ltd,Hohhot,Inner Mongolia 010030,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院/农业部饲草栽培、加工与高效利用重点实验室/教育部草地资源重点实验室,内蒙古呼和浩特010018 [2]蒙草生态环境(集团)股份有限公司,内蒙古呼和浩特010030

出　　处：《草地学报》2023年第8期2334-2342,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金(32160326);内蒙古自治区种业科技创新重大示范工程“揭榜挂帅”项目(2022JBGS0014);内蒙古自治区直属高校基本科研业务费种业振兴专项(BR22-11-10)资助。

摘　　要：‘蒙农杂种’冰草(Agropyron cristatum×A.desertorum‘Hycrest-Mengnong’)具有干草产量高,适口性好,抗逆性强的特点,是中国北方干旱地区建立人工草地的适宜草种。本研究利用同源克隆方法从‘蒙农杂种’冰草中克隆得到的1个MYB类转录因子基因(命名为AcdMYB 1),对其进行了生物信息学分析和表达分析。结果表明:该基因cDNA序列长度为1161 bp,包含完整开放阅读框(ORF)为969 bp,编码322个氨基酸,具有MYB转录因子的保守结构域。AcdMYB1与小麦族的山羊草、普通小麦、野生二粒小麦的同类基因亲缘关系最近,序列一致性均在90%以上。组织表达中AcdMYB1基因在穗中的表达量最高,其次为叶、根,茎中表达量最低;在模拟自然干旱胁迫下,能够显著降低AcdMYB 1的表达水平。烟草亚细胞定位显示,该蛋白定位于细胞核。本研究为进一步探索‘蒙农杂种’冰草AcdMYB 1基因及MYB转录因子家族提供了科学依据。Agropyron cristatum×A.desertorum‘Hycrest-Mengnong’is a pasture variety cultivated by Inner Mongolia Agricultural University and it a tetraploid grass(2n=28)with high hay yield,good palatability and strong resistance to stress.It is a suitable grass species for establishing artificial grassland in arid areas of north China.A MYB transcription factor gene was cloned from A.cristatum×A.desertorum‘Hycrest-Mengnong’by homology-based cloning,named as AcdMYB 1,the bioinformatics analysis and expression analysis were carried out on.The results showed that a cDNA sequence with a length of 1161 bp and a maximum open reading frame of 969 bp encoding 322 amino acids,the AcdMYB 1 sequence had a conserved domain of MYB transcription factor.AcdMYB 1 had a genetic relationship with Aegilops tauschii,Triticum aestivum and Triticum dicoccoides by more than 90%sequence homology.The expression analysis results showed that the expression of AcdMYB 1 gene was the highest in the spike,followed by leaves,roots and stems.Under a drought stress,the expression level of AcdMYB 1 was significantly reduced.The protein was localized in the nucleus.The study provided a scientific basis for further research on the AcdMYB 1 gene and the MYB transcription factor family in A.cristatum×A.desertorum‘Hycrest-Mengnong’.

关 键 词：蒙农杂种冰草 基因克隆 表达分析 MYB转录因子 

分 类 号：Q785[生物学—分子生物学] Q786