Optimization of Extraction Conditions for Total Flavonoids from Fructus Aurantii Immaturus and Its Anti-UVB Radiation Activity  

作　　者：Xu CHANG Yanhui WANG Ximing LIU Yunge MA Yunhao LIU Xiaobo CHEN Mengyao SUN Liyan LI 

机构地区：[1]Medical School,Huanghe Science and Technology College,Zhengzhou 450063,China [2]College of pharmacy,Henan University,Kaifeng 450046,China

出　　处：《Agricultural Biotechnology》2023年第4期110-114,118,共6页农业生物技术（英文版）

基　　金：Supported by National Key Program of Innovation and Entrepreneurship Training for College Students (202211834021);Project Funds of Zhengzhou Science and Technology Bureau (ZZSZX202109).

摘　　要：[Objectives]Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus(TFFAI)and its resistance activity to ultraviolet radiation were investigated in present research.[Methods]The optimal extraction conditions of TFFAI were determined by single factor and orthogonal experiments,and the survival rate of TFFAI on HaCaT cells irradiated with UVB rays was investigated.It s antioxidant capacity was determined by ABTS.[Results]The results showed that the highest yield of TFFAI was obtained with 70%ethanol at a solid-to-liquid ratio of 1:50(w/v)and 40℃for 1.5 h by single-factor and orthogonal experiments.Total flavonoids(0.25-1.00 mg/ml)could significantly improve the survival rate of HaCaT cell line.Meanwhile,the maximum absorption peak of TFFAI was found at 283 nm,and in-vitro antioxidant experiment identified that TFFAI had a good clearance rate to ABTS.It suggestes that TFFAI could protect the cells from UVB damage by absorption of UVB rays and anti-oxidation.[Conclusions]TFFAI played a protective role on UVB irradiated cells through UVB ultraviolet absorption and antioxidant pathways.

关 键 词：Fructus Aurantss Immaturus Total flavonoids Optimization of extraction conditions UVB radiation resistance 

分 类 号：R284.2[医药卫生—中药学]