丙泊酚通过miR-182-5p介导HIF-1α通路对缺氧诱导胎盘滋养细胞生物学活性的影响  

Effect of propofol regulating HIF-1α signaling pathway through miR-182-5p on biological activity in placental trophoblast cells induced by Hypoxia

作　　者：孙艺娟[1] 贾杰[1] 邓恋[1] 漆冬梅[1] 陈祥楠[1] 黎昆伟[1] 王培宗[2] SUN Yijuan;JIA Jie;DENG Lian;QI Dongmei;CHEN Xiang-nan;LI Kunwei;WANG Peizong(Department of Anesthesiology,Guangdong Women and Children′s Hospital,Guangzhou 511400,China;不详)

机构地区：[1]广东省妇幼保健院麻醉科,广州511400 [2]中山大学肿瘤防治中心麻醉科,广州510060

出　　处：《实用医学杂志》2023年第15期1869-1875,共7页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金项目(编号:B2021238);广州市科技计划项目(编号:201904010460)。

摘　　要：目的 探讨丙泊酚通过miR-182-5p介导HIF-1α通路对缺氧诱导胎盘滋养细胞生物学活性的影响。方法 采用缺氧诱导体外培养的HTR-8/SVneo细胞,丙泊酚干预,采用CCK-8检测细胞的活力、Transwell测定细胞迁移和侵袭,TUNEL检测细胞凋亡。通过qRT-PCR测量miR-182-5p和HIF-1α相对mRNA表达水平。Western blot检测相关通路蛋白表达水平。结果 与对照组比较,缺氧组细胞迁移和侵袭能力降低,细胞凋亡率升高(P <0.05),miR-182-5p、MMP-9表达水平降低,HIF-1α、VEGF表达水平升高(P <0.05)。与缺氧组比较,丙泊酚干预后,细胞迁移、侵袭能力恢复,细胞凋亡率降低(P <0.05),miR-182-5p、MMP-9表达水平升高,HIF-1α、VEGF表达水平降低(P <0.05)。转染抑制剂后则逆转了丙泊酚的治疗作用。结论 丙泊酚通过调节miR-182-5p/HIF-1α轴改善缺氧诱导的HTR-8/SVneo细胞毒性。Objective To study the effect of propofol regulating HIF-1α signaling pathway through miR-182-5p on biological activity in placental trophoblast cells induced by Hypoxia.Methods Hypoxia-induced in vitro cultured HTR-8/SVneo cells were used in this study.Cells were treated with propofol.CCK-8 was used to detect cell viability;Transwell to determine cell migration and invasion,and TUNEL to detect apoptosis.The relative mRNA expression level of miR-182-5p and HIF-1α was measured by qRT-PCR.Western blot was used to detect the expression level of related pathway proteins.Results Compared with the control group,the hypoxic group showed reduced cell migration and invasion ability,increased apoptosis rate(P<0.05),reduced miR-182-5p and MMP-9 expression level,and increased HIF-1α and VEGF expression level(P<0.05).Compared with the hypoxic group,propofol intervention restored cell migration and invasion ability,decreased apoptosis rate(P<0.05),increased miR-182-5p,MMP-9 expression level,and decreased HIF-1α and VEGF expression level(P<0.05).Transfection with inhibitors reversed the therapeutic effect of propofol.Conclusion Propofol ameliorates hypoxia-induced HTR-8/SVneo cytotoxicity by regulating the miR-182-5p/HIF-1α axis.

关 键 词：丙泊酚 人绒毛外滋养细胞 先兆子痫 miR-182-5p/HIF-1α轴 

分 类 号：R392.5[医药卫生—免疫学]