2型糖尿病合并骨质疏松患者血清miR-210、miR-30a、miR-16的变化及影响因素分析  

Changes of serum miR-210,miR-30a and miR-16 in patients with type 2 diabetes mellitus combined with osteoporosis and analysis of the influening factors

作　　者：王强 商金祥 陈雪荣[2] Wang Qiang;Shang Jinxiang;Chen Xuerong(Departmend of Ornbopedics,Affiliated Hospital of Shaoxing College of Arts and Scienes,Shaoxing 312000,China;Department of Orthopedics,Shaoxing People's Hospital,Shaoxing 312000,China)

机构地区：[1]绍兴文理学院附属医院骨科,绍兴312000 [2]绍兴市人民医院骨科,绍兴312000

出　　处：《中华内分泌外科杂志》2023年第4期459-463,共5页Chinese Journal of Endocrine Surgery

基　　金：浙江省医药卫生科技计划项目(2022KY1313)。

摘　　要：目的探究2型糖尿病(type2diabeticmellitus,T2DM)合并骨质疏松患者血清miR-210、miR-30a、miR-16的变化及分析影响因素。方法前瞻性选取2019年5月至2022年5月绍兴文理学院附属医院骨科收治的T2DM患者80例为研究对象,按患者是否合并骨质疏松将患者分为T2DM合并骨质疏松患者42例、T2DM未合并骨质疏松患者38例。另选取同期本院体检健康者40例为对照组。比较各组miR-210、miR-30a、miR-16的差异,并通过R0C曲线分析miR-210、miR-30a、miR-16对T2DM合并骨质疏松的诊断价值,并分析T2DM合并骨质疏松影响因素。Spearman相关性分析miR-210、miR-30a、miR-16与T2DM合并骨质疏松相关指标的关系。结果各组间miR-210、miR-30a、miR-16比较差异有统计学意义(F=24.13、62.69、307.26,P<0.05),对照组<T2DM未合并骨质疏松组<T2DM合并骨质疏松组(P<0.05)。R0C曲线分析显示,miR-210、miR-30a、miR-16诊断T2DM合并骨质疏松的AUC依次为0.779、0.854、0.973,均诊断有一定准确性。二元Logistic多因素回归分析显示,T2DM病程、糖化血红蛋白(glycosylatedhemoglobin,HbAlc)、I型胶原羧基端肽β特殊系列(β-CTX)均为T2DM合并骨质疏松的危险因素(P<0.05),身体质量指数(bodymass index,BMI)、甲状旁腺激素(parathyroidhormone,PTH)、25羟基维生素D3(25-hydroxyvitaminD3monohydrate,25(OH)D,)、骨钙素(boneGlaprotein,BGP)、骨密度(bonemineraldensity,BMD)均为T2DM合并骨质疏松的保护因素(P<0.05)。且Spearman相关性分析显示:miR-210、miR-30a、miR-16与BCP、BMD存在负相关(r=-0.668/-0.592/-0.599、-0.671/-0.609/-0.593),与β-CTX存在正相关(r=0.670/0.603/0.605)。结论miR-210、miR-30a、miR-16在T2DM合并骨质疏松患者血清中高表达,T2DM病程、HbA1c、β-CTX均为T2DM合并骨质疏松的危险因素,BMI、PTH、25(OH)D3、BGP、BMD均为T2DM合并骨质疏松的保护因素。Objective To investigate the changes of serum miR-210,miR-30a and miR-16 in patients with type 2 diabetes mellitus(T2DM)combined with osteoporosis(OP)and to analyze the influencing factors.Methods Eighty patients with T2DM admitted to our hospital from May.2019 to May.2022 were prospectively se-lected for the study,and the patients were divided into 42 patients with T2DM combined with OP and 38 patients with T2DM without OP according to whether the patients were combined with OP.Another 40 cases were selected as the control group during the same period of physical examination.We compared the differences of miR-210,miR-30a and miR-16 among the groups,and analyzed the diagnostic value of miR-210,miR-30a and miR-16 on T2DM combined with OP by ROC curve,and analyzed the influencing factors of T2DM combined with OP by binary logis-tic regression.miR-210,miR-30a and miR-16 were analyzed by Spearman correlation.Spearman correlation analy-sis of miR-210,miR-30a,miR-16 and T2DM combined OP related indexes.Results The differences of miR-210,miR-30a,and miR-16 were statistically significant between groups(F=24.13,62.69,307.26,P<0.05),and the con-trol group<T2DM uncomplicated OP group<T2DM combined OP group(P<0.05).ROC curve analysis showed that miR-210,miR-30a,and miR-16 The AUCs for the diagnosis of T2DM combined with OP were 0.779,0.854 and 0.973 in order,all of which had some accuracy in diagnosis.Binary logistic multi-factor regression analysis showed that the duration of T2DM disease,glycosylatedhemoglobin(HbAlc),type I collagen carboxy-terminal peptide β special series(β-CTX)were all risk factors for T2DM combined with OP(P<0.05),body mass index(BMI).BMI,parathyroid hormone(PTH),25-hydroxyvitamin D3monohydrate(25(OH)D3),bone Gla protein(BGP),bone mineral density(BMD)were all risk factors for T2DM combined with OP(P<0.05).Spearman correlation analysis showed that miR-210,miR-30a and miR-16 were negatively correlated with BGP and BMD(r=-0.668/-0.592/-0.599,-0.671/-0.609/-0.593)and positively correlated with β-CTX(r=0

关 键 词：2型糖尿病 骨质疏松 MIR-210 miR-30a miR-16 

分 类 号：R587.1[医药卫生—内分泌] R580[医药卫生—内科学]