不同抗凝剂对富血小板血浆释放生长因子及引起SD大鼠注射疼痛的影响比较  

Comparison of effects of different anticoagulants on the release of growth factor and injection pain of platelet-rich plasma in SD rats

作　　者：陈莉 刘广亚 许育兵 朱展鸿 李艳辉 单桂秋 周谋 CHEN Li;LIU Guangya;XU Yubing;ZHU Zhanhong;LI Yanhui;SHAN Guiqiu;ZHOU Mou(Department of Blood Transfusion,General Hospital of Southern Theater Command,Guangzhou 510010,China)

机构地区：[1]南部战区总医院输血医学科,广东广州510010

出　　处：《中国输血杂志》2023年第8期660-664,共5页Chinese Journal of Blood Transfusion

基　　金：南部战区总医院院内科技计划项目(2021NZA009)。

摘　　要：目的探讨不同抗凝剂对富血小板血浆(platelet-rich plasma,PRP)释放生长因子含量及引起注射疼痛的影响比较。方法选取无偿献血者15名,每位献血者同时使用4种抗凝管(EDTA-K2抗凝、EDTA-NA2抗凝、枸橼酸钠抗凝、ACD-A抗凝管),分为4组,使用富浆法分离制备PRP,应用酶联免疫吸附试验法检测激活后的PRP中血小板源性生长因子-AA(platelet derived growth factor AA,PDGF-AA)、转化生长因子-β(transforming growth factor-β,TGF-β)、胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1),血管内皮生长因子(vascular endothelial growth factor,VEGF)和血小板因子4(platelet factor 4,PF-4)的含量;同时使用4组制备的PRP及PG上清液与对照组生理盐水对SD大鼠(20只,4只/组)进行皮下或者皮内注射。观察并记录SD大鼠在注射过程中的疼痛状态,依据美国实验动物疼痛指南评估5组实验动物的疼痛状态。结果PRP中血小板计数比较:D组最高(1294.53±277.37)×10^(9)/L,与A组(789.13±377.13)×10^(9)/L和C组(990.94±493.12)×10^(9)/L比较,P<0.05,差异具有统计学意义;生长因子检测OD值比较:A、B、C、D,4组PDGF-AA的OD值为:1.51±0.18、1.69±0.21、0.66±0.19、1.72±0.13,组间差异具有统计学意义(P<0.05),D组优于其他3组;PF-4的OD值为:1.18±0.24、1.61±0.14、0.65±0.26、1.72±0.10,组间差异具有统计学意义(P<0.05),D组优于其他3组;IGF-1检测的OD值为:1.02±0.08、0.98±0.11、1.06±0.11、1.32±0.65,组间差异无统计学意义(P>0.05);VEGF检测的OD值为:0.13±0.04、0.21±0.14、0.08±0.02、0.13±0.04,B、C 2组间差异具有统计学意义(P<0.05);TGF-β检测的OD值为:0.14±0.01、0.15±0.01、0.28±0.17、1.10±0.37,组间差异具有统计学意义(P<0.05),D组优于其他3组;动物注射疼痛感比较:注射PRP和PG上清液时,A、B、C、D,4组与对照组生理盐水比较差异均具有统计学意义(P<0.05);注射PRP时,A、B、C、D,4组的疼痛评分的中位数为:6(1.5)、5(0.75)、4.5(2.5)Objective To investigate the effects of different anticoagulants on platelet-rich plasma(PRP)release content of growth factor and injection pain.Methods A total of 15 voluntary blood donors were selected,with each blood donor using four kinds of anticoagulant tubes with EDTA-K2 anticoagulation,EDTA-NA2 anticoagulation,citrate anticoagulation,ACD-A anticoagulation respectively as group A,B,C and D.PRP was isolated and prepared by the rich plasma method,and the contents of PDGF-AA,TGF-β,IGF-1,VEGF,and PF-4 were detected by enzyme-linked immunosorbent assay.Meanwhile,SD rats(20,4/group)were injected subcutaneously or intradermally with the supernatant of PRP and PG gel prepared in the 4 groups and normal saline in the control group.The pain status of SD rats during the injection was observed and recorded.The pain status of the 5 groups of experimental animals was evaluated according to the American Laboratory Animal Pain Guide.Results The platelet counts in PRP in group D was the highest[(1294.53±277.37)×10^(9)/L],which was sig-nificantly higher than that in group A[(789.13±377.13)×10^(9)/L]and group C[(990.94±493.12)×10^(9)/L](P<0.05).The OD value of PDGF-AA in group A,B,C,and D were 1.51±0.18,1.69±0.21,0.66±0.19 and 1.72±0.13,respectively,with statistically significant difference between groups(P<0.05)and group D better than the other three groups.The OD value of PF4 was 1.18±0.24,1.61±0.14,0.65±0.26 and 1.72±0.10 respectively,with statistically significant difference between groups(P<0.05)and group D better than other three groups.The OD value of IGF-1 was 1.02±0.08,0.98±0.11,1.06±0.11 and 1.32±0.65 respectively,with no significant difference be-tween groups(P>0.05).The OD value of VEGF was 0.13±0.04,0.21±0.14,0.08±0.02 and 0.13±0.04 respectively,with statistically significant difference between group B and C(P<0.05).The OD value of TGF-βwas 0.14±0.01,0.15±0.01,0.28±0.17 and 1.10±0.37 respectively,with statistically significant difference between groups(P<0.05)and group D better than o

关 键 词：抗凝剂 富血小板血浆 PLT计数 生长因子 注射疼痛 

分 类 号：R457.14[医药卫生—治疗学] R973.2[医药卫生—临床医学] R331.343