自体富血小板血浆治愈儿童难治性骨髓炎合并骨折1例  

Autologous platelet-rich plasma cured refractory osteomyelitis complicated with fracture:a case report

作　　者：刘建香[1,2] 吕孟兴 陈昊[3] 高立兰 胡美坤[1,2] 单桂秋[4] 屈柯暄 LIU Jianxiang;LYU Mengxing;CHEN Hao;GAO Lilan;HU Meikun;SHAN Guiqiu;QU Kexuan(Department of Blood Transfusion,Kunming Children′s Hospital,Kunming 650228,China;Yunnan Children′s Health and Disease Clinical Medicine Research Center;Department of Orthopaedics,Kunming Children′s Hospital;Department of Blood Transfusion Medicine,Southern War Zone General Hospital;Kunming Children′s Blood Disease Immune and Molecular Diagnostic Medical Technology Center)

机构地区：[1]昆明市儿童医院输血科,云南昆明650228 [2]云南省儿童健康与疾病临床医学研究中心 [3]昆明市儿童医院骨科 [4]南部战区总医院输血医学科 [5]昆明市儿童血液疾病免疫与分子诊断医学技术中心

出　　处：《中国输血杂志》2023年第8期673-675,共3页Chinese Journal of Blood Transfusion

基　　金：云南省昆明市卫生科技人才培养项目暨“十百千”工程[2021-SW(省)-024];云南省儿童健康与疾病临床医学研究中心开放课题(2022-ETYY-YJ-09);昆明市儿童血液疾病免疫与分子诊断技术中心[2022-SW(技术)-007]。

摘　　要：目的自体富血小板血浆(platelet rich plasma,PRP)联合治疗成功治愈儿童难治性骨髓炎合并骨折的经验分享。方法经传统治疗14月余无效的儿童难治性骨髓炎合并骨折1例,采集患儿全血20 mL,应用二次离心法制备4~5倍浓度PRP共6 mL,3 mL PRP与0.3 mL激活剂混合,制备浓缩血小板凝胶(PG)2 cm×2 cm覆盖骨折端,剩余3 mL PRP与0.3 mL激活剂使用三通管喷洒到周围组织。结果患者随访1周X线片显示骨折线模糊,骨折端有明显骨痂形成;4个月X线片复查,骨折端愈合良好,骨折面愈合,骨髓炎痊愈。结论应用自体PRP联合治疗儿童难治性骨髓炎合并骨折具有一定的临床效果,可为儿童难治性骨髓炎治疗提供新思路。Objective To share the experience of autologous platelet-rich plasma(PRP)combined therapy in successful treatment of refractory osteomyelitis with fractures in children.Methods One case of refractory osteomyelitis with fracture in children failed to respond to traditional treatment for more than 14 months.A total of 20 mL of whole blood was collected from the child,and 6 mL of PRP with 4 to 5 times concentration was prepared by secondary centrifugation.To prepare 2 cm×2 cm platelet concentrate gel(PG),3 mL of PRP was mixed with a 0.3 mL activator which was then covered with an absorbable dressing.A three-way tube sprayed the remaining 3 mL of PRP and 0.3 mL activator into the surrounding tissues.Results The X-ray film of the patient followed up for 1 week showed that the fracture line was blurred,and the fracture end had obvious callus formation.The X-ray film reexamination at 4 months showed that the fracture end healed well,the fracture surface healed,and the osteomyelitis healed.Conclusion Autologous PRP has a good effect in the treatment of refractory osteomyelitis combined with fracture in children,which can provide a new method for clinical treatment.

关 键 词：儿童 骨髓炎 骨折 自体富血小板血浆 血浆 

分 类 号：R457.14[医药卫生—治疗学] R331.143[医药卫生—临床医学] R551.3