烟台地区HBsAg单试剂反应性献血者追踪检测与归队结果分析  

Following up and re-entry of HBV reactive blood donors in Yantai area

作　　者：宋和蔚 沈欣堂 王玮 徐明华 SONG Hewei;SHEN Xintang;WANG Wei;XU Minghua(Yantai Central Blood Station,Yantai 264003,China)

机构地区：[1]烟台市中心血站,山东烟台264003

出　　处：《中国输血杂志》2023年第8期713-716,共4页Chinese Journal of Blood Transfusion

摘　　要：目的分析烟台地区乙型病毒肝炎(HBV)血液筛查及追踪检测数据,探讨HBV反应性献血者追踪检测与归队策略的合理性。方法对血液筛查酶联免疫吸附试验(ELISA)单试剂反应性且核酸扩增检测技术(NAT)无反应性的献血者追踪检测。追踪检测采用单人份核酸检测(ID-NAT)的方法检测HBV DNA,ELISA检测乙型肝炎表面抗原(HBsAg)、乙型肝炎病毒表面抗体(抗-HBs)、乙型肝炎病毒e抗体(抗-HBe)、乙型肝炎病毒e抗原(HBeAg)及乙型肝炎病毒核心抗体(抗-HBc),电化学发光免疫分析法(ECLIA)检测HBsAg。结果ELISA血液筛查HBsAg单试剂反应性且HBV DNA无反应性献血者547名。追踪献血者97名,24名符合归队条件,73名不符合归队条件。符合归队条件的24名献血者中13名再次献血,检测结果全部合格。结论采用ELISA、ID-NAT及ECLIA 3种方法联合追踪检测HbsAg单试剂反应性献血者,血清学抗-Hbs S/CO≥10且其他检测结果无反应的献血者实施归队处理。归队率24.74%,归队后献血者再次献血合格率100%。Objective To analyze the preliminary screening and follow-up testing data of HBV in Yantai area,and discuss the rationality of following up and re-entry program of HBV reactive blood donors.Methods Donors who were single reagent reactive by enzyme-linked immunosorbent assay(ELISA)in initial screening but non-reactive by nucleic acid testing(NAT)were followed up.Individual NAT(ID-NAT)was performed for HBV DNA,ELISA for HBsAg,HBsAb,HBeAb,HBeAg and HBcAb,and ECLIA for the detection of HBsAg.Results A total of 547 blood donors were HBsAg ELISA-/NAT+,and 97 were followed up,among which 24 met the requirements of re-entry while 73 did not.Of the 24 blood donors who met the re-entry requirements,13 donated blood again,with test results all qualified.Conclusion The combination of ELISA,ID-NAT,and ECLIA methods for following up detection for HBsAg ELISA+blood donors is recommended.Blood donors with HbsAb S/CO≥10 and negative results for other tests met the re-entry requirements,with a re-entry rate at 24.74%,and the re-donation qualified rate of blood donors after re-entry was 100%.

关 键 词：HBV 献血者归队 核酸检测 追踪检测 ECLIA 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学]