草莓果生刺盘孢菌全基因组候选效应蛋白预测及功能分析  

Whole-genome Prediction and Functional Analysis of Colletotrichum fructicola Candidate Effector Proteins in Strawberry

作　　者：朱家君 段可[2] 高清华[2] 续益荣 倪迪安 杨静 Zhu Jiajun;Duan Ke;Gao Qinghua;Xu Yirong;Ni Di'an;Yang Jing(School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai,201418;Forest and Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403)

机构地区：[1]上海应用技术大学生态技术与工程学院,上海201418 [2]上海市农业科学院林木果树研究所,上海201403

出　　处：《分子植物育种》2023年第17期5631-5641,共11页Molecular Plant Breeding

基　　金：上海市科委农业科技重点攻关项目(20392001500);上海市农业科学院2022年度农业科技创新支撑项目(JCYJ222201)共同资助。

摘　　要：胶孢炭疽菌(Colletotrichum gloeosporioides)复合种下果生刺盘孢(C.fructicola)引起的草莓炭疽病严重危害草莓产业,病原菌通过分泌效应蛋白协助侵染宿主。为了研究果生刺盘孢效应蛋白与草莓互作机理,本研究通过生物信息学对草莓果生刺盘孢的效应蛋白进行了全基因组预测,并对所得候选蛋白进行序列分析。结果表明,共获得355个候选效应蛋白,分属于29个已知蛋白家族;转录组测序结果显示260个候选效应蛋白在侵染草莓后的24、72和96 h均显著上调表达,60个蛋白在三个时间点均下调表达;对候选效应蛋白进行了蛋白互作网络分析,获得两个蛋白互作模块,发现其参与宿主细胞壁裂解和调控菌株致病力。本研究为探究草莓炭疽病的致病机理研究提供了分子靶标。Strawberry anthracnose caused by Colletotrichum fructicola(C.fructicola)of the species complex(C.gloeosporioides)sensu lato,seriously endangers the strawberry industry.The pathogen intends to infect the host by secreting multiple effector proteins.In order to clarify the protein interaction mechanism between C.gloeosporioides and strawberry,genome-wide prediction of effector proteins in C.gloeosporioides was conducted by bioinformatics,and the obtained candidate sequences were further screened and analyzed.In total 355 candidate effectors were finally harvested,which belonged to 29 known protein families.Transcriptomic sequencing results showed that the expression of 260 candidate effector proteins were among them were significantly up-regulated at 24,72 and 96 h post strawberry inoculation,and 60 proteins were down-regulated at these three time points.The candidate effector proteins were analyzed by protein interaction network,and two protein interaction modules were obtained,and it was found that they were involved in host cell wall lysis and regulation of strain pathogenicity.This research provided crucial molecular targets for exploring the pathogenesis of strawberry anthracnose.

关 键 词：草莓 果生刺盘孢(Colletotrichum fructicola) 全基因组 效应蛋白 蛋白互作 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]