基于LC-MS技术对镉胁迫下地钱代谢组的性别响应差异研究  

Study on gender response difference of Marchantia polymorpha metabolome under cadmium stress based on LC-MS technique

作　　者：何静 易航 杨希 荣姝恬 王丽[1] HE Jing;YI Hang;YANG Xi;RONG Shu-Tian;WANG Li(College of Life Sciences,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学生命科学学院,成都610065

出　　处：《四川大学学报（自然科学版）》2023年第4期159-168,共10页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技计划项目(川林规函[2021]959号-002)。

摘　　要：为探究雌雄异株植物响应重金属胁迫的性别差异,本研究利用超高效液相色谱结合四级杆飞行时间质谱仪(UPLC-Q-TOF-MS)测定了镉(Cd)胁迫下雌雄地钱(Marchantia polymorpha)中的代谢产物,分析了与不同性别相关的差异代谢物和代谢通路.结果显示,未受镉胁迫时,雌性地钱中类黄酮生物合成、嘌呤代谢和玉米素生物合成代谢水平高于雄性,芹菜素、木犀草素、腺嘌呤和腺苷含量显著高于雄性.受镉胁迫后,雌性地钱中类黄酮生物合成、嘌呤代谢和玉米素生物合成代谢水平下降,芹菜素、木犀草素、矢车菊素-3-O-半乳糖苷、腺嘌呤和腺苷含量显著下降.而雄性地钱中上述三条通路的代谢水平上升,刺槐苷、槲皮素、腺苷一磷酸和肌苷的含量显著上调.研究表明,地钱响应镉胁迫的代谢变化出现了性别差异.其中,雌性地钱降低了玉米素和遗传物质的合成水平,诱导谷胱甘肽的合成.雄性地钱上调了玉米素合成,缓解叶绿素的降解,同时上调类黄酮生物合成途径中的槲皮素和刺槐苷等具有抗氧化活性的次生代谢产物.In order to explore the gender differences of dioecious plants in response to heavy metal stress,the metabolites of Marchantia polymorpha under cadmium(Cd)stress were determined by ultra-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS),and the differential metabolites and metabolic pathways related to different genders were analyzed.The results showed that,without cadmium stress,the levels of flavonoid biosynthesis,purine metabolism and zeatin biosynthesis in females were higher than those in males with significantly higher contents of apigenin,luteolin,adenine and adenosine.After cadmium stress,the levels of flavonoid biosynthesis,purine metabolism and zeatin biosynthesis in females were decreased with the significant decrease of apigenin,luteolin,cyanidin-3-O-galactoside,adenine and adenosine.The metabolic levels of the above three pathways increased in males with the significant increase of differential metabolites kaempferol-3-O-robinoside-7-O-rhamnoside,quercetin,adenosine monophosphate and inosine.Studies have shown gender differences in metabolic changes of M.polymorpha in response to cadmium stress.Among them,female M.polymorpha reduced the synthesis level of zeatin and genetic material,and induced the synthesis of glutathione.Male M.polymorpha increased zeatin synthesis,alleviated chlorophyll degradation,and up-regulated secondary metabolites with antioxidant activity such as quercetin and kaempferol-3-O-robinoside-7-O-rhamnoside in the flavonoid biosynthesis pathway.

关 键 词：镉胁迫 地钱 性别响应差异 代谢组 

分 类 号：Q945[生物学—植物学]