南海海绵Dactylospongia elegans的二倍半萜类化学成分及抗炎活性研究  

Study on the sesterterpenoids of Dactylospongia elegans from the South China Sea and their anti-inflammatory activities

作　　者：康永锋[1,2,3] 武改芳 李立 甘建红[1] KANG Yong-feng;WU Gai-fang;LI Li;GAN Jian-hong(School of Food Science,Shanghai Ocean University;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation;National Experimental Teaching Demonstration Center for Food Science and Engineering,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院 [2]上海水产品加工及贮藏工程技术研究中心 [3]食品科学与工程国家级实验教学示范中心,上海201306

出　　处：《天然产物研究与开发》2023年第8期1357-1363,共7页Natural Product Research and Development

基　　金：上海市“浦江人才”计划项目(2020PJD082);上海市“科技创新行动计划”自然科学基金面上项目(20ZR1470600)。

摘　　要：利用溶剂分步萃取、正相硅胶柱层析、反相ODS柱层析和高效液相等多种色谱技术对中国南海海绵Dactylospongia elegans的化学成分进行分离纯化,通过波谱学手段并结合文献比对鉴定化合物的结构。从二氯甲烷萃取部位分离鉴定了8个化合物:dactylospene F(1)、scalarin(2)、honulactone A(3)、honulactone B(4)、honulactone E(5)、honulactone F(6)、honulactone I(7)、honulactone J(8),其中化合物1为新化合物,化合物2~8为首次从该海绵中分离得到。对8个化合物的抗炎活性进行了评价,结果显示化合物1、3、4在10μmol/L时对脂多糖诱导的一氧化氮生成有较好的抑制作用,抑制率分别为65.5%、48.5%、46.0%,且对小鼠巨噬细胞RAW 264.7无细胞毒性。Solvent extraction,silica column chromatography,ODS column chromatography and HPLC methods were used to isolate and refine compounds from the marine sponge Dactylospongia elegans from the South China Sea.The chemical structures were identified by spectroscopic analysis and comparison with the literature.From the CH_(2)Cl 2 extract of D.elegans,eight compounds were isolated and determined as dactylospene F(1),scalarin(2),honulactone A(3),honulactone B(4),honulactone E(5),honulactone F(6),honulactone I(7),honulactone J(8).Compound 1 was a new compound and compounds 2-8 were isolated from D.elegans for the first time.All of the isolates were evaluated for their anti-inflammatory activity.The results showed that compounds 1,3 and 4 exhibited inhibitory effects on nitric oxide production induced by lipopolysaccharide at 10μmol/L,with inhibition rate of 65.5%,48.5%and 46.0%,respectively,and showed no cytotoxicity to RAW 264.7 in mouse macrophages.

关 键 词：Dactylospongia elegans 二倍半萜 抗炎活性 NO产生 

分 类 号：R284[医药卫生—中药学] R915[医药卫生—中医学]